大流行流感疫苗血清学检测中马血球和鸡血球血凝抑制试验方法的比较

Comparison of horse and chicken red cells-based hemagglutination inhibition(HI) assays for serological evaluation of pandemic influenza vaccines

血凝抑制试验(HI)是评价季节性流感疫苗免疫效果的经典方法。由于对人、禽流感病毒的受体不同,不同来源的红血球检测敏感性可能有差异。实验中比较了经典的鸡血球HI方法和国外报道的马血球HI方法,发现两种方法在检测大流行流感疫苗接种者血清时,于不同毒株抗原检测时表现各不相同,检测结果差异在2倍之内,说明经典的鸡血球HI方法仍适用于评价大流行流感疫苗的免疫效果。

The hemagglutination-inhibition （HI） assay is a traditional method for assessing immune responses to influenza virus. It is well known that the receptors for avian and human influenza virus on the host cells are different; therefore, HI test based on red ceils from various species of animals containing different receptors may have different sensitivity. In this study, The traditional chicken red cells-based HI （ cHI ） with horse red ceils based-HI （hHI ） methods was compared in the detection of antibodies against H5N1 influenza virus. It found that two kinds of method have different performances when 3 kinds of HI antigens were used, but the differences between two assays were roughly within the 2-fold range. Therefore, the traditional chicken red ceils-based HI is still suitable for the serological evaluation of pandemic influenza vaccines.