炭末明胶改良安瓿法应用研究

A applied study of improved charcoal powder ampoul method

为了证实炭末明胶改良安瓿管法替代其他方法检测细菌是否产明胶酶,实验中采用营养明胶法、X线胶片法和炭末明胶改良安瓿管法对486株质控菌株、临床分离菌株进行明胶液化试验。结果显示,以营养明胶法培养的359株呈阳性反应,X线胶片法274株呈阳性反应,炭末明胶改良安瓿法423株呈阳性反应。营养明胶法明胶液化平均天数为3.3d,而炭末明胶改良安瓿法明胶液化平均天数仅为1.5d。炭末明胶改良安瓿法由于简单快捷,易于观察,而且试剂用量小、能够室温长期保存,该方法值得推广应用。

To study whether gelatinase can be produced by bacteria with improved charcoal powder gelatin arnpoul method. Gelatin liquefaction tests were performed for 486 quality-controlling bacteria strains and clinically isolated bacteria strains with the nutrition gelatin method, x-ray film method and improved charcoal powder gelatin ampoul method, respectively. The results showed that 359 strains are positive with nutrition gelatin method, 274 strains are positive with the x-ray film method ,423 strains are positive with improved charcoal powder gelatin ampoul method. The average day of gelatin liquefaction is 3.3 days with nutrition gelatin method and 1.5 days with improved charcoal powder gelatin ampoul method. More easy and more rapidly, improved charcoal powder ampoul method is worth to extend because of little reagents and stored at room temperature for long-time conservation.