摘要
目的探讨参附注射液(ShenfuInjection)对大鼠全脑缺血再灌注时核转录因子-κB(nuclear factor kappaB,NF-κB)表达的影响及其治疗作用。方法采用四血管阻塞的方法复制出大鼠全脑缺血再灌注模型,采用免疫组化法、原位杂交法和原位末端标记法分别检测假手术组(A组)、全脑缺血再灌注组(B组)、参附注射液治疗组(C组)海马CA1区NF-κB、肿瘤坏死因子-αmRNA(TNF-αmRNA)表达及细胞凋亡数的变化。结果与假手术组比较,全脑缺血再灌注组海马CA1区NF-κB和TNF-αmRNA的表达及细胞凋亡数明显增加(P<0.01);NF-κB和TNF-αmRNA的表达分别于再灌注6h和12h达到高峰,并持续到48h;细胞凋亡数随再灌注时间的延长而逐渐增多(P<0.01)。参附注射液治疗后NF-κB和TNF-αmRNA的表达下降,细胞凋亡数减少(P<0.01)。结论在脑缺血再灌注救治过程中参附注射液可能通过抑制NF-κB与TNF-αmRNA的表达,进而减少细胞凋亡而发挥脑保护作用。
Objective To explore the protective effects of Shenfu injection on rats with global cerebral ischemia-reperfusion. Methods The model of global cerebral ischemia - reperfusion was established by four - vessel occlusion method. Using immunohistochemical technique, in situ hybridization and in situ end labeling technique （TUNEL）, the expressions of NF-κB and TNF-amRNA and the number of the apoptotic cell were examined in the sham-operated group （group A）, the control group （the group after global cerebral ischemia reperfusion treated with Saline, group B） and the group treated with Shenfu injection（the group after global cerebral ischemia reperfusion treated with Shenfu injection, group C） separately. Results Compared with group A, the expressions of NF-κB and TNF-amRNA in group B increased significantly （P〈0. 01 ）, which peak at 6h and 12h respectively. With the time of reperfusion prolonged, the number of apoptotic cell gradually increased （P〈0. 01）. Compared with group B, the expressions of NF-κB and TNF-amRNA and the number of apoptotic cell in group C decreased （P〈0. 01）. Conclusions Shenfu injection may down-regulate the expressions of NF-κB and TNF-amRNA after global cerebral ischemia - reperfusion. It attenuated cellular apopto sis.
出处
《卒中与神经疾病》
2008年第6期363-366,共4页
Stroke and Nervous Diseases
