SOCS-1、SOCS-3、TNF-a在有机磷中毒鼠肝脾的表达

Study on the expression of SOCS-1 and SOCS-3,TNF-a in liver and spleen of multiple organ dysfunction syndrome mice induced by acute organophosphorus pesticide poisoning

目的观察有机磷中毒(AOPP)小鼠肝脏、脾脏SOCS-1、SOCS-3及TNF-amRNA的表达,探讨其在AOPP致多器官功能障碍综合症(MODS)中的发病机制,以期为MODS的防治提供新的策略。方法健康小鼠随机分成敌敌畏组、生理盐水对照组和正常对照组,分别于染毒后2、6、12、24h取肝脾组织,以逆转录聚合酶链反应(RT-PCR)测定SOCS-1、SOCS-3、TNF-a表达水平。结果AOPP小鼠肝脾SOCS-1、SOCS-3、TNF-a表达均升高,与对照组比较差异有统计学意义(P均<0.05)。结论AOPP可导致细胞因子TNF-a失调,可能参与了AOPP后SIRS向MODS的发病过程;若能抑制炎症反应的失控,则对中毒MODS的治疗将有新的突破。

Objective To investigate the dynamic of SOCS-1.SOCS -3 TNF-α in live and spleen of MODS mice induced by acute organophosphorus pesticide poisoning （AOPP）,and discuss the mechanism of MODS induced by AOPP. Methods The mice were randomly：divided into AOPP group. And control group .and the liver and the spleen tissue were taken in 2 hours ,6 hours ,12 hours and ,24 hours after exposed to poison for pathological test as well as RNA and protein.The expression of regulatory genes SOCS-1 .SOCS- 3 and TNF-α were detected by RT-PCR. Results The levels of SOCS-1 . SOCS- 3 and TNF-α increased obviously in the AOPP group ,and there was significant difference between mices in AOPP group and normal control group （P〈0.01）. Conclusion AOPP may lead to the maladjustment of the cytokines ,and the cytokines TNF-α play an important role in the development- from systemic inflammatory response （SIRS）to MODS in AOPP. SOCS can be induced by TNF-α in the liver and the spleen , Considering SOCS can self-regulate , if uncontrolled cytokine can be inhibited.