荧光PCR对从业人员肠道致病菌筛查的评价

Evaluation of real-time PCR in detecting enteric pathogens

目的评价荧光PCR法用于食品及公共场所从业人员肠道致病菌的筛查。方法随机采集12780份从业人员肛拭子标本用荧光PCR检测,以传统分离培养方法作为"金标法"进行对照,分析灵敏性和特异性。结果在12780份标本中,荧光PCR检测出沙门菌阳性14份,阳性率为1.10‰,培养法检测出10份,阳性率为0.78‰,沙门菌荧光PCR检测法的灵敏度为100%,特异性为99.97%。荧光PCR检测出志贺菌18份,阳性率为1.41‰,培养法检测出3份,阳性率为0.23‰,志贺菌荧光PCR法的灵敏度为100%,特异性为99.88%。结论荧光PCR法灵敏度性高,特异性强,简便快速,为从业人员健康检查提供了一套快速的筛查方法,值得广泛推广应用。

Objective To evaluate the results of real-time PCR in detecting enteric pathogens in practitioners in the fields of food and public service. Methods There 12 780 samples were determined by real-time PCR comparing with traditional culture as golden standard method. The sensitivity and specificity were analyzed. Results In 12780 samples, 14 and 10 samples were positive by real-time PCR and culture for Salmonella respectively. The positive rate was 1.10‰ and 0.78‰. The sensitivity and specificity were 100% and 99.97%. 10 were positive by culture PCR for Solmonella with a positive rate of 0.78‰. There 18 and 3 samples were positive by real-time PCR and culture for Shigella respectively. The positive rate was 1.41‰ and 0.23‰. The sensitivity and specificity were 100% and 99.88%. Conclusion Real-time PCR is of high sensitivity and high specificity and it is a simple and fast method for rapid screening enteric pathogens in practitioners in the fields of food and public service.