318株血培养分离病原菌的分布及耐药性分析

Distribution and drug resistance of 318 pathogenic bacteria strains isolated from cultured specimens

目的了解2005～2007年我院血培养分离菌株的构成比及耐药情况。方法采用BacT Alert 120全自动血培养仪及VITEK-AMS全自动微生物鉴定仪对血培养阳性标本进行菌种鉴定和药物敏感试验。结果3 216份血标本中,检出细菌318株,检出率为9.89%,革兰阳性菌占56.6%;革兰阴性菌占37.1%;念珠菌属占6.3%。甲氧西林耐药株和甲氧西林敏感株对抗生素的耐药谱差异有显著性(P<0.01),而革兰阴性杆菌对亚胺培南、头孢哌酮/舒巴坦和阿米卡星的敏感性较高。结论革兰阳性球菌为本院引起茵血症的主要病原茵,凝固酶阴性葡萄球菌占优势。定期进行细菌耐药性的监测,有助于临床合理应用抗菌药物。

Objective To investigate distilbution and drug resistance pathogenic bacteria isolated from blood samples in this hospital during the period of 2005-2007. Methods Blood samples were cultivated with Bact Alert-120 automated hemanalysis system and pathogenic bacteria were identified wlth Vitek-AMS instrument. Results Totally 318 strains of bacteria were isolated from 3 216 blood samples with a ,positive rate of 9.89%.Gram-postitve bacteria were dominant （56.6% ）, Graru-negtive bacteria were accounting for 37.1% ,Candida strains were accounted for 6.3%. The resistance rate showed significant differences between the methicillin-resistant staphylococcus （MRS） and methicillin-sensitive Staphylococcus （MSS） （P〈0.01）. Gram-negative bacilli were more sensitive to imipenem, cefoperazone/sulbactam and amikacin. Conclusion Gram-posfitve microorganisms are the main pathogenic bacteria and coagulase negative Staphylococcus （CNS） predominated. Monitoring the drug resistance of bacteria regularly is helpful for reasonable clincal application of anfibacterials.