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银环蛇线粒体基因组全序列分析 被引量:5

Analysis of Mitochondrial Genome of Bungarus multicinctus
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摘要 根据GenBank公布的蛇类物种线粒体基因序列和已知的引物序列,总共设计和合成了9对引物.采用保真度较高的Ex-Taq酶,以总基因组DNA为模板进行PCR扩增,产物纯化后进行TA克隆和步移测序,拼接后获得了全长17144bp银环蛇线粒体基因组全序列.其共编码13种蛋白质、2种rRNA和22种tRNA.这些基因没有内含子,基因间排列紧密,仅有极少或完全没有核苷酸,甚至相互重叠.除了含有2个调控线粒体基因组复制和转录的控制区外,其余基因在长度和位置等方面与其它脊椎动物均具有较高的同源性.
出处 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2008年第12期1177-1182,共6页 Chinese Journal of Biochemistry and Molecular Biology
基金 全军医药卫生科研基金资助项目(No.2006166125) 广东省自然科学基金资助项目(No.06019716)~~
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共引文献22

同被引文献76

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