卡波金对急性声损伤耳蜗外侧壁微血管的影响

The effects of carbogen inhalation on microvasculars within lateral wall of cochlear following acute acoustic trauma

目的:观察卡波金(carbogen)对豚鼠急性噪声性声损伤后耳蜗外侧壁微血管的影响,探讨卡波金在声损伤早期治疗中的应用价值。方法:纯白健康豚鼠40只随机分为单纯噪声暴露组、单纯吸入卡波金组、噪声暴露加卡波金吸入组以及空白对照组。每组实验动物均为10只。应用活体显微镜技术,观察豚鼠噪声暴露和(或)吸入卡波金后耳蜗外侧壁微血管的变化情况,通过对微血管红细胞流柱宽度(RBC column diameter,RBC-CD)、血流速度(blood flow velocity,BFV)、血流流态(blood flow states,BFS)的描述反映耳蜗外侧壁局部微循环的变化。结果:空白对照组耳蜗外侧壁微血管血流稳定,单纯噪声暴露组可见方向相反的逆向血流,血管内可见成簇状的细胞聚集现象。与单纯噪声暴露组比较,噪声暴露加卡波金吸入组逆行血流减少。单纯吸入卡波金组RBCCD与对照组比较增加20.7%,单纯噪声暴露组较对照组RBCCD减少12.1%,组间差异有统计学意义(P<0.05)。噪声暴露加卡波金组较单纯噪声暴露组RBCCD增加17.4%。单纯吸入卡波金组血流呈线流或线粒流;空白对照组和噪声暴露加卡波金吸入组血流呈线粒流或粒线流。单纯噪声暴露组血流呈粒流、粒缓流甚至出现粒摆流。结论:吸入卡波金气体后耳蜗外侧壁血管RBCCD明显增加,并使血液流速加快。在急性声损伤的早期干预中,吸入卡波金对耳蜗微循环的改善是一种有积极意义的措施。

Objective：To explore the influence of carbogen on lateral wail microvasculars of cochlear after acute acoustic trauma. Method：Forty guinea pigs were divided into 4 groups： group A （noise damage）, group B （carbogen inhalation）, group C （noise damage ＋ carbogen inhalation）, and the control group without any treatment. The diameter of the column of RBCs（RBC column diameter,RBCCD）,blood flow velocity（BFV） and blood flow states（BFS） in microvasculature were measured and described under microscope. Result： The microvascularis in group A demonstrated a blood flow in contrary direction, granuliform flow, and granular slow flow. The erythrocytes aggregated in the mierovascularis of the cochlea. The RBCCD decreased 12.1% compared with the control group（ P 〈0.05）. The blood flow in group B showed a laminar flow or laminar granular flow, and the RBCCD increased 20.7% compared with the control group. The blood condition in group C was the same as the control group-laminar granular blood flow; the blood flow with contrary direction was less than group A, and the RBCCD was 17.4% lager than that of group A. Conclusion：Carbogen can dilate the RBCCD and increase the BFV in stria vascularies. So carbogen can alleviate the harm from noise.