非小细胞肺癌中survivin表达及其与bcl-2表达的关系

Expression of survivin protein and its relationship with expression of bcl-2 protein in non-small-cell lung cancer

目的：探讨非小细胞肺癌（non-small-cell lung cancer，NSCLC）组织中survivin蛋白表达及其与bcl-2蛋白表达的相关性。方法：应用免疫组织化学方法检测60例非小细胞肺癌组织及15例正常肺组织中survivin蛋白的表达，分析其与NSCLC临床病理参数之间的关系，并探讨其与bcl-2蛋白的表达的相互关系。结果：60例NSCLC组织中，survivin蛋白的阳性表达率为61．7％（37／60），15例正常肺组织中无survivin蛋白表达（P〈0．05）。survivin蛋白的表达与肺癌TNM分期、淋巴结转移和细胞分化程度有密切关系（P〈0．05），但与患者年龄、性别、吸烟与否及肿瘤组织学类型无关（p〉0．051。此外。survivin与bcl-2蛋白的表达具有相关性（P〈0．05）。结论：survivin蛋白在肺癌组织中高表达并与肺癌TNM分期、淋巴结转移和细胞分化程度有密切关系，提示该基因对NSCLC的发生发展起重要作用。survivin基因有望成为肺癌基因治疗的新靶点并可作为判断病情和评价预后的指标。survivin蛋白表达与bcl-2蛋白表达呈正相关．二者在非小细胞肺癌的发生中可能起协同作用。

Objective： To study the expression of survivin protein in non-small-cell lung cancer and its relationship with the expression of bcl-2 protein. Methods： Expression of the Survivin proteins was examined by immunohistochemical method in 60 non-small-cell lung cancer tissues and 15 normal lung tissues. Study its relationship with clinicopathological features and expression of bcl-2 protein in NSCLC. Results： Survivin proteins was expressed in 37 of 60 （61.7%） cases of non-small-cell lung cancer. In contrast, normal lung tissue did not express survivin proteins（P〈0.05）. The positive expression of survivin proteins was associated with TNM stages, lymph node involvement,cell differentiation（P〈0. 05）, but not associated with ages, gender, smoking, histological classification（P〉0.05）. The expression of survivin correlated with bcl-2（P〈0.05）. Conclusion： The positive expression of survivin proteins correlated with TNM stages, lymph node involvement, cell differentiation in NSCLC suggested that survivin may play a role in the pathway of carcinogenesis and may be identified as a potential therapeutic target in NSCLC. It might be used to evaluate prognosis. The close relationships between the expression of survivin and bcl-2 proteins indicate that they might play synergetic roles in the process of carcinogenesis of NSCLC.