摘要
目的：建立大枣中无刺枣苄苷Ⅱ的HPLC分析方法。方法：采用ZorbaxSB—C18（4．6mm×250mm，5μm）色谱柱，以甲醇-水（20：80）为流动相，流速为1mL·min^-1，检测波长为210nm，柱温30℃。结果：无刺枣苄苷Ⅱ在0．046—0．582μg呈良好的线性关系，r=0．9999。12批不同产地的大枣中无刺枣苄苷Ⅱ质量分数在0.013％～0．041％。结论：该方法简单、重复性好，可用于大枣的质量控制。
Objective： To develop a quantitative method for determination of zizybeoside Ⅱ in Ziziphus jujuba. Method： The samples were separated at 30℃ on a Zorbax SB-C18 column eluted with methanol-water （20： 80） as the mobile phase. Flow rate was set at 1.0 mL ·min^-1 and the detection wavelength was set at 210 nm. Result： The calibration curve was linear within the range from 0. 046 to 0. 582 μg （r =0. 999 9） and the average recovery was 97.2%. 12 batches of the crude drugs purchased from different areas were determined and the contents of zizybeoside Ⅱ in Fructus Jujubae were fluctuated from 0. 013% to 0. 041%. Conclusion： The method is simple, repeatable and could be used for the quality control of Z. jujuba.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第24期2935-2937,共3页
China Journal of Chinese Materia Medica
基金
国家科技支撑计划项目(2006BAI08B03-09)
