银翘散在不同煎煮时间下对免疫抑制小鼠的免疫调节作用

Immune-Regulating Effect of Yinqiao Powder in Different Decocted Time on Rats with Immune Suppression

目的通过观察银翘散在不同煎煮时间下对免疫抑制小鼠免疫功能的影响探讨银翘散的最佳煎煮时间。方法将88只小鼠随机分为11组:空白对照组,模型对照组,3分钟高、中、低浓度组,6分钟高、中、低浓度组,12分钟高、中、低浓度组。除空白组外均用环磷酰胺造成免疫抑制模型,银翘散灌胃给药7天后测定各组小鼠网状内皮系统对血流中惰性炭粒的吞噬廓清能力、游泳耐力时间、外周血中T细胞亚群、脾脏和胸腺器官重量。结果3分钟高浓度组、3分钟中浓度组、6分钟高浓度组可显著提高免疫抑制小鼠的游泳耐力时间(P<0.05),3组比较差异无统计学意义;除6分钟低浓度组、12分钟中、低浓度组外,其余各组均能显著增强免疫抑制小鼠网状内皮系统的吞噬功能、K值和α值均明显增高(P<0.05),其中以3分钟高浓度和6分钟高浓度的效果为优;银翘散可显著提高各组小鼠外周血CD4+T细胞的数量(P<0.05),银翘散各组有升高小鼠外周血CD8+T细胞数量的趋势,但作用不显著;3分钟高浓度组能显著性增加各组小鼠胸腺重量和脾脏重量(P<0.05)。结论银翘散对免疫抑制小鼠具有免疫增强作用,其效果以煮沸后3分钟效果为佳。

Objective To explore the best decocted time by observing the effect of Yinqiao Powder （Lonicerae and Forsythiae Powder） in different decocted time on immunity of rats with immune suppression. Methods The 88 qualified male rats were randomly divided into 11 groups with 8 in each., normal control group, model group, groups of Yinqiao Powder decocted for 3 minutes in high, medium and low concentrations, groups of Yinqiao Powder decocted for 6 minutes in high, medium and low concentrations, and groups of Yinqiao Powder decocted for 12 minutes in high, medium and low concentrations. Except for the normal control group, the rats in all other groups were made immune suppression models by using cyclophosphamide. Yinqiao Powder decoction was given by intragastric administration for 7 days. Then, the carbon clearance ability of reticuloendothelial system, the swimming endurance, the quantity of T cell subpopulation in peripheral blood, and the weights of spleen and thymus of all groups were determined. Results Yinqiao Powder decocted for 3 minutes in high and medium concentrations and decocted for 6 minutes in high concentration could improve remarkably the swimming endurance （P〈0.05）, without significant difference among these 3 groups. Except for those decocted for 6 minutes in low concentration and decocted for 12 minutes in medium and low concentrations, the other groups of Yinqiao Powder could improve remarkably the phagocytosis of reticuloendothelial system, and the K and a values were increased remarkably （P〈0.05）, of which, the effect of those decocted for 3 minutes in high concentration and decocted for 6 minutes in high concentration was the best. Yinqiao Powder could increase remarkably the quantity of CD4^＋ T cell of peripheral blood （P〈0.05）.There was a tendency in Yinqiao Powder groups in increasing CD8^＋ T cell of peripheral blood, but not remarkably. Yinqiao Powder decocted for 3 minutes in high concentration could increase remarkably the weight of thymus and spleen （P〈0.05）. Conclusion Yinqiao Powder is effective in improving immunity in rats with immune suppression and the effect of that decocted for 3 minutes was the best.