兰州地区大气悬浮颗粒物对人全血细胞DNA合成的影响

The Effect of the Total Suspended Particulates (TSP) in Atmospheric Air on DNA Synthesis in Human Blood Cells in Lanzhou

为探讨兰州市大气总悬浮颗粒物（ＴＳＰ）对细胞ＤＮＡ损伤修复的影响。检测了ＴＳＰ对人全血细胞ＤＮＡ合成影响，采用程序外ＤＮＡ合成（ＵＤＳ）试验方法，结果表明，各检测点ＴＳＰ在加与不加Ｓ９条件下均具有一定的致突变活性（Ｐ＜０．０５或Ｐ＜０．０１），且各点ＵＤＳ测定值均呈较好的剂量反应关系。说明ＵＤＳ方法检测大气ＴＳＰ混合物致突性有一定应用价值。

To explore the effect of total suspended particulate(TSP)in air on the damage and repairement of DNA in cells,human blood cells treated by extract of TSP were determined with unscheduled DNA synthesis assay in vitro.The results showed that all five tested TSP had mutagenicity with or without the activation of S9(P<005或P<001))and a better doseresponse relationship could be found.It indicated that UDS presented avialable for detecting the mutagenicity of TSP in atmospheric air.