骨髓间充质干细胞治疗早期骨性关节炎的实验研究

An experimental study on treatment of early osteoarthritis with BMSCs transplantation

目的:用骨髓间充质干细胞(BMSCs)治疗兔早期骨性关节炎(OA),探讨其可行性及疗效评价。方法:将72只新西兰大白兔随机分成对照组、OA组和BMSCs治疗组,每组24只。治疗组在造模后第4周左膝关节腔内注射BMSCs。观察动物的一般情况,TNF-α和IL-1β水平,MRI扫描,取膝关节标本行大体及病理观察并进行Mankin’s评分。结果:与OA组相比,BMSCs治疗组术后步态逐渐好转;血清TNF-α和IL-1β水平降低;MRI示低信号区缩小,关节软骨厚度接近正常,无关节腔积液;大体标本见关节软骨表面趋于平坦完整,有软骨覆盖,但光泽欠佳;病理检查提示有透明样软骨形成,细胞排列整齐,厚薄基本一致,软骨相连。结论:BMSCs关节腔内可成软骨分化,为早期OA患者提供了一种新的有效疗法。

Objective：To analyze the effects of BMSCs transplantation on early osteoarthritis in rabbits. Methods：Seventy-two New Zealand white rabbits were randomly and equally divided into normal control group, early osteoarthritis control group and BMSCs transplantation group. Injection of BMSCs was given to the rabbits in the transplantation group on the fourth week after arthritis induction. The general information of animals,serum TNF-α and IL-1β levels,MRI,gross morphological and pathohistological changes were observed as well as Mankin scores. Results：Compared with the early osteoarthritis group,the rabbits of BMSCs group had a better gait;the serum TNF-α and IL-1β levels were decreased;MRI showed the diminution of low signal area,the thickness of articular cartilage got close to normal and without arthroedema; The surface of articular cartilage became evenness by gross morphology but the luster below the mark;histopathology indicated hyalo-chondrogenesis and to line up in order with equal thickness. Conclusion：BMSCs can induced into chondrocytes in articular cavity and be effective for early osteoarthritis in rabbits.