益肾健脾胶囊对人工髋关节松动影响的细胞因子学研究

YiShenJianPi granule's effect on cytokine in loosening of prothesis

目的探讨益肾健脾胶囊对人工髋关节松动的影响。方法随机选择准备行人工髋关节置换术患者20例。采集外周血,分离单个核细胞,每例标本分成5份。A组:仅单个核细胞,为对照组;B组:单个核细胞+骨水泥微粒;C组:单个核细胞+骨水泥微粒+益肾健脾胶囊(3mg/ml)。D组:单个核细胞+骨水泥微粒+益肾健脾胶囊(6mg/ml)。E组:单个核细胞+骨水泥微粒+益肾健脾胶囊(12mg/ml)。培养48h后,用ELISA测定细胞上清液中肿瘤坏死因子(TNF)-a、白细胞介素(IL)-6含量。结果B组的TNF-a和IL-6含量均明显高于A组(P<0.01);C组的TNF-a和IL-6含量明显低于B组(P<0.05)。结论骨水泥微粒可刺激单个核细胞分泌TNF-a和IL-6,而益肾健脾胶囊能有效地抑制单个核细胞分泌TNF-a和IL-6。益肾健脾胶囊能防治人工关节置换术后由TNF-a和IL-6等溶骨性细胞因子引起的假体周围的骨溶解,因此该制剂有望成为防治人工关节松动的有效药物。

Objective To explore the effects of YiShe,JianPi granule on aseptic eytokine of loosening of prothesis in vitro. Methods From 20 patients, blood sample were taken, whose mononuclear cells were isolate and then divided into five groups. Group A：only mononuclear cells, which was control gronp;gronp B： mononuclear cells ＋ PMMA particles; Group C： mononuelear cells ＋ PMMA particles ＋ YiShenJianPi granule（3mg/ml）; Group D： mononuclear cells ＋ PMMA particles ＋ YiShenJianPi granule（6mg/ml）;Group E： rnononuclear cells ＋ PMMA particles ＋ YiShenJianPi granule（ 12mg/ ml）. They were cultured for 48 hours, and the a mount of osteolytic factors such as TNF - α and IL - 6 of each group was determined. Results The levels of TNF - α and IL - 6 were much higher in group B than A, C, D and E（ P 〈 0.01 ）. The levels of TNF - α and IL - 6 were much lower in group C, D and E than B（ P 〈 0.05）. Conclusion It shows the PMMA particles could stimulate manonuclear cells to excrete TNF - α and IL - 6, which can be inhibited by YiShenJianPi granule. By inbiting bone resorption associated with the particles and TNF- α and IL- 6, YiShenJianPi granule may have clinical application in the prevention and treatment of aseptic loosening of prothesis.