茜草醇提物对小鼠U14宫颈癌抑制作用的研究

Effect of RCL on mice bearing in U14 cervical cancer

目的:探讨茜草醇提物(RCL)抗肿瘤作用及机制。方法:检测RCL的抑瘤作用;血细胞分析仪对小鼠红细胞数(RBC)、白细胞数(WBC)和血小板(PLT)进行检测,全自动生化分析仪对小鼠血清的谷草转氨酶(AST)、谷丙转氨酶(ALT)、尿素氮(BUN)和肌酐(Cr)水平进行测定;流式细胞术检测细胞周期和细胞凋亡。结果:RCL灌服剂量为500 mg/kg和1000 mg/kg能不同程度抑制小鼠U14宫颈癌实体瘤的生长和延长U14小鼠的生存时间,肿瘤抑制率分别是47.68%和58.94%,生命延长率分别为47.92%和76.70%;阻止肿瘤细胞于G1期;RCL对小鼠造血系统、肝功和肾功没有影响。结论:RCL对小鼠U14宫颈癌具有一定抑制作用,其作用机制可能与抑制肿瘤细胞周期有关。

Objective： Effect of RCL on anti--tumor and mechanism. Method：The anti--tumor effect of RCL was tested, the number of red blood cells （RBC） in mice,WBC and platetet （PLT） were tested by hema- tology analyzer,the serum aspartate aminotransferase （AST）,alanine aminotransferase （ALT）, urea Nitrogen （BUN） and creatinine （Cr） levels were determined by fully automatic biochemical analyzer; cell cycle and apoptosis were analyzed by flow eytometry. Resuits：RCL （500mg/kg and 1000mg/kg） could inhibit mice bearing in U14 solid tumor growth and prolong the survival time,the inhibition rate up to 47. 68% and 58. 94%, increasing rate in life to 47. 92% and 76.70%. RCL could stop tumor cell in G1 phase without hematopoietic system, liver and kidney injury. Conclusion：RCL has some inhibition effect on mice bearing in U14 cervical cancer and its mechanism might be related to tumor cell cycle inhibited.