正交设计优化茴香随机扩增多态DNA反应体系的研究

Study on Optimization of the RAPD System of Fennel by Orthogonal Design

目的建立茴香的随机扩增多态DNA(RAPD)最佳反应体系。方法正交设计及方差分析应用于建立RAPD反应体系的研究。结果茴香的RAPD最佳反应体系为25μl的反应体系中,含10×Buffer 2.5μl,2.0 m MdNTPs 3.0μl,2.0 U/μlTaq酶1.0μl,2.0μmol/L随机引物4.0μl,30.0 ng/μl模板DNA30 ng,ddH2O 13.5μl。结论正交设计及方差分析适用于茴香RAPD反应体系的建立。

Objective To establish optimal RAPD reaction system of Fennel. Methods The orthogonal design and variance analysis were used to optimize the RAPD system. Results The optimal Fennel RAPD system was composed of 2.5 μl 10 × PCR Buffer, 3.0 μl dNTPs（2.0 mmol/L）, 1.0 μl TaqE（2.0 U/μl ） ,4. 0μl Primer（2.0 μmol/L）, 1.0 μl DNA （30.0 ng/μl ）, 13.5 μl ddH2 0 with total 25 μl reaction system. Conclusion The orthogonal design and variance analysis can be used to optimize the RAPD system.