高效液相色谱法测定旱莲草中木犀草素和芹菜素的含量

HPLC Determination of Luteolin and Apigenin in Herba Ecliptae

目的建立了同时测定旱莲草中木犀草素和芹菜素含量的高效液相色谱（HPLC）法。方法采用Shim-pack CLC-ODS色谱柱（150mm×6mm，5μm），流动相为乙腈-2％的醋酸水溶液（体积比为30：70），流速1．0ml·min6^-1，检测波长340nm，柱温为40℃。结果在上述条件下，木犀草素和芹菜素的质量浓度分别在0．003～0．24mg·ml^-1和0．0025～0．20mg·ml^-1时与色谱峰面积之间的线性关系良好，两者的平均加标回收率分别为100．6％和102．5％。结论该方法快速、简便、准确，可用于旱莲草中木犀草素和芹菜素的HPLC测定。

Objective To establish an HPLC method for content determination of luteolin and apigenin in Herba Ecliptae. Methods HPLC separation was carried out in a Shim -pack CLC -ODS columm （150 mm 6 mm,5p, m） with acetonitrile -2% acetic acid （30： 70, v/v） as mobile phase at a flow rate of 1.0 ml · min ^-1. The detection wavelength was 340 nm and the column temperature was maintained at 40℃. Results There were good linear relationships between the mass concentrations and the peak areas of luteolin and apigenin in the ranges of 0. 003 - 0.24 mg · ml^-1 and 0. 0025 - 0.20 mg · ml ^- 1 , respectively. The recovery rates were 100.6% and 102.5% , respectively. Conclusion This method is rapid, accurate and reproducible for determination of luteolin and apigenin in Herba Ecliptae.