荆防散干预一氧化氮合酶-一氧化氮通路的抗炎机制实验研究

Study on the Mechanism Related to the NOS/NO Pathway of Anti-inflammatory Action of JingFang San

目的观察荆防散干预炎症模型动物一氧化氮合酶-一氧化氮(NOS/NO)通路的抗炎机制,为其功效和临床应用提供药理学依据。方法采用角叉菜胶诱导大鼠胸膜炎模型和卵白蛋白致小鼠哮喘模型,测定大鼠胸腔渗出液与小鼠肺组织匀浆中NOS,iNOS活力和NO含量的变化。结果荆防散各剂量组能不同程度地降低小鼠肺组织匀浆与大鼠胸腔渗出液中NOS、iNOS活力,并减少NO含量,其中以5 g.kg-1剂量作用显著。结论荆防散能通过抑制NOS活力,尤其是与炎症反应密切相关的iNOS活力,从而减少炎症介质NO的生成来发挥抗炎作用,提示干预NOS/NO通路是其抗炎作用机制之一。

Objective To investigate the mechanism related to NOS/NO pathway of anti - inflammatory action of JingFang San, and to pharmacological basis for its efficiency and clinical use of treating allergic inflammation disease. Methods Inflammated ani- mal model were carried out by the carrageenan - induced pleurisy in rats and ovabumin - induced asthma in mice. Pleurorrhea in rats and lung tissue homogenate in mice were prepared for the detection of NOS activity and NO level. Results Compared with model group, JingFang San at the doses of 5,2.5 and 1.25 g · kg^-1 could decrease the NOS activity and the NO level in inflammated area in different degree, and the suppressive effect was most significant at the dose 5g · kg^-1. Conclusion JingFang San has anti -inflammatory action in two animal inflammation models, which mechanism may be related to suppressing NOS activity, especially iNOS, and decrease the NO level.