摘要
目的:探讨prostasin基因在卵巢癌组织中的表达及意义。方法:①应用4 097靶基因点基因芯片对5例卵巢浆液性腺癌及5例正常卵巢组织进行基因表达谱分析,筛查差异表达基因。②应用RT-PCR检测10例卵巢癌组织、10例卵巢瘤组织及10例正常卵巢组织中prostasin mRNA的表达。结果:①基因芯片筛查出卵巢浆液性腺癌差异表达基因58个,其中表达增高(上调趋势)基因30个,表达降低(下调趋势)基因28个;prostasin基因为表达上调基因(cy5/cy3=3.018)。②prostasin基因在卵巢癌组织中表达显著增高(prostasin/actin:0.415±0.177),与正常卵巢组(prostasin/actin:0.192±40.128)及卵巢瘤组(prostasin/actin:0.237±0.232)比较无显著性差异(P>0.05)。结论:基因芯片可以用于筛查卵巢癌差异表达基因,prosta-sin为表达上调基因,prostasin mRNA在卵巢癌组织中表达显著高于在正常卵巢及卵巢瘤组织中表达。
Objective： To study the expression of prostasin in ovary cancer and its significances. Methods： （1）Gene microarray containing 4 097 genes was used to analyze gene expression patterns in tissue samples from 5 cases of human ovarian serous cystadenocarinoma and 5 eases of normal ovarian. （2）RT - PCR method was used to investigate the expression of prostasin mRNA in tissues samples of 10 eases ovarian cancer, 10 cases benign ovarian tumor and 10 eases normal ovarian. Results： （1）58 genes of differential expression were found in ovarian cancer, the expression of 30 genes increased （ up - regulated）, the expression of 28 genes decreased （ down - regulated）, prostasin was the up - regulated gene （ Cy5/Cy3 = 3. 018）.（2）The level of prostasin mRNA expression in ovary cancer tissues （ prostasin/aetin： 0. 415 ±0. 177） was significantly higher than that in normal ovary tissues （prestasin/aetin： 0. 192 ±0. 128） and behign ovary tumor tissues （ prostasin/aetin： 0. 237 ± 0. 232 ） （ P 〈 0. 05） . Conclusion： Gene microarray could screen differential expression gene in ovary cancer; prostasin is up - regulated gene; the expression of prostasin mRNA is signifieandy higher than that of normal and benign ovary tumor tissues.
出处
《中国妇幼保健》
CAS
北大核心
2008年第36期5195-5197,共3页
Maternal and Child Health Care of China
基金
吉林省教育厅资助课题(吉教科合字[2005]第132号)
