摘要
目的:探讨鸦胆子油乳对大鼠异位及在位的子宫内膜中半胱天冬酶-3(caspase-3)表达的影响,为鸦胆子油乳用于临床治疗子宫内膜异位症提供理论依据。方法:以雌性Wistar大鼠为研究对象,用自体移植法制作子宫内膜异位症模型,造膜成功后用鸦胆子油乳腹腔给药治疗,观察异位囊肿变化情况,并用免疫组化及蛋白印迹法检测子宫组织中caspase-3的表达情况。结果:各组标本caspase-3蛋白的表达均呈阳性,治疗组异位及在位子宫内膜组织中caspase-3表达均高于模型组。结论:鸦胆子油乳能抑制大鼠异位内膜的生长,其作用机制可能是通过上调caspase-3的表达,促进异位内膜细胞的凋亡实现的。
Objective： To investigate the influence of bruceolic oil emulsion on caspase -3 in eutopic and ectopic endometrium of rat with endometriosis, to offer evidence for the therapy of endometriosis with bruceolic oil emulsion. Methods： Femina rats were specimen, taking peritoneal injection with brueeolle oil emulsion after the endometriosis mode was set up by surgery of self endometrium transplant, the change of ectopie focus was observed and the expression of caspase - 3 in the endometrium was detected by immunohistochemistry and Western blotting. Results： Protein was found in all specimens and the expression of caspase - 3 in ectopic and eutopic endometrium of treated group was more than that of control group. Conclusion： Bruceolic oil emulsion could inhibit the growth of the ectopic endometrium in rats with experimentally induced endometriosis. The therapeutic mechanism may be related to promoting the apeptosis in ectopic endometrial tissue by increasing caspase -3 expression.
出处
《中国妇幼保健》
CAS
北大核心
2008年第36期5198-5201,共4页
Maternal and Child Health Care of China
基金
黑龙江省教育厅科学技术研究项目(项目编号:11511356)
