牙刷刷毛黏附口腔黏膜脱落细胞DNA的检验方法

A Method for DNA Examination on Mouth Mucosa Exfoliative Cells from Tooth-Brush Bristles

目的探索牙刷刷毛黏附口腔黏膜脱落细胞DNA提取有效方法。方法采用牙刷刷毛拔出法和直接涮洗法收集口腔黏膜脱落细胞,分别用Chelex-100法和DNAIQ试剂盒提取DNA进行PCR扩增及STR分型。结果牙刷刷毛拔下后收集口腔黏膜脱落细胞检测STR9个以上基因座成功率明显高于直接涮洗法,差异具统计学意义(P<0.05)。Chelex-100法和DNAIQ试剂盒提取法之间差异无统计学意义(P>0.05)。结论牙刷刷毛拔出法较直接涮洗法收集口腔黏膜脱落细胞更有效。

Objective To establish a method for DNA examination on mouth mucosa exfoliative cells from toothbrush bristles. Methods Slough-off mouth mucosa exfoliative cells were collected by bristles extracted method and direct washing method. The DNA of exfoliative cells was extracted with Chelex-100 method and DNA IQTM kit. Then all the extracted DNA took PCR amplification and STR analysis. Results There was a significant difference between the groups with bristles extracted method and with direct washing method at the success rates of over 9 STR loci detected （P〈0.05）. However, there was no statistical significance between Chelex-100 method and DNA IQTM kit （P〉0.05）. Conclusion It is demonstrated that mouth mucosa exfoliative cells collected by bristles extracted method is more available than that with direct washing method.