“碘泵基因”转染胶质瘤细胞后摄碘能力的研究

The study of radioiodide uptake by transfered the sodium/iodide symporter gene into gliomas

目的:证明转染人类钠碘转运体(hNIS)基因入胶质瘤后,胶质瘤细胞可以摄取放射性碘。方法:使用脂质体转染法利用重组质粒将hNIS基因转染入人胶质瘤细胞系U251中,使胶质瘤细胞获得hNIS基因;经过新霉素抗生素筛选获得稳定表达hNIS的细胞系,然后进行体外摄125I实验及过氯酸盐抑制实验;体外125I反流实验,体外125I内流实验,并绘制时间-每分钟放射性计数曲线。结果:在使用质粒转染hNIS基因到U251细胞中后,成功地获得稳定表达hNIS基因的细胞系hNIS-U251。hNIS-U251细胞系可以摄取碘,而且这种摄碘的功能是由hNIS基因所介导的,转染后的细胞摄碘能力可以提高117倍左右。结论:在细胞系hNIS-U251中,放射性碘元素可以被胶质瘤细胞大量摄取。

Objective： To transfect hNIS gene into human glioma cell lines U251 by recombinant expression plasmids and observe radioiodine intaken by U251. Methods： hNIS gene was transfected into human glioma cell lines U251 by recombinant expression plasmids with lipofectamine 2000-plasmid complexes. The stably expressing hNIS gene cell lines （hNIS-U251）were selected with G418. Subsequently we investigated stably expressing hNIS gene cell lines （hNIS-U251）＇s biologic function, including ^125I uptake assay of stably expressing hNIS gene cell lines, ^125I influx-course, ^125I-efflux-course. Results： We transfected hNIS gene into human glioma cell lines by recombinant expression plasmids successfully, and obtained the stably expressing hNIS gene cell lines： hNIS-U251. The hNIS-U251 cell lines could intake radioactive iodide by hNIS gene. The uptakes of ^125I were 117 fold higher in hNIS-U251 cell lines than in U251 cell lines. Conclusion： The stably expressing hNIS gene hNIS-U251 cell lines can intake radioiodine.