应用FQ-PCR检测宫颈病变中HPV 16/18亚型DNA

HPV 16/18 DNA quantitative analysis of cervical lesions by fluorescence quantitative PCR

目的:探讨荧光定量聚合酶链反应(FQ-PCR)检测宫颈病变人乳头瘤病毒(HPV)16/18亚型DNA的可行性,建立快速、灵敏的检测方法,指导宫颈癌的普查和治疗。方法:选择124例阴道细胞学检测异常的宫颈脱落细胞,使用FQ-PCR方法检测HPV16/18亚型DNA,同时取宫颈病变处组织进行组织病理学检查。结果:124例样本中HPV16/18亚型DNA阳性者43例,阳性率为34.68%;DNA定量范围在5.4×102 copies/ml～6.38×107 copies/ml,平均为5.75×105 copies/ml;组织病理学检测27例阳性,阳性率为21.77%;经χ2检验,二者有显著性差异(χ2=10.2272,P<0.05);阴性标本为0 copy/ml。结论:FQ-PCR方法检测宫颈病变HPV 16/18亚型DNA感染,具有快速、简便、灵敏度高、特异性强等优点,对宫颈癌的普查和治疗有指导意义。

Objective： To evaluate FQ-PCR on quantitative detection HPV 16/18 DNA in cervical lesions. Methods： HPV16/18 DNA were detected in 124 csess of cervical lesions by FQ-PCR. HPV was also detected by histopathological examination. Results： The positive rate of HPV 16/18 DNA was 34.68% by FQ-PCR. The copies of HPV 16/18 DNA were 5.4 × 10^2 copies/ml-6.38 × 10^7 copies/ml,average was 5.75 × 10^5copies/ml. The positive rate of HPV was 21.77% by histopathologieal examination. There was a significant difference between FQ-PCR and histopathological examination.There were 0 copy/ml in negative cases. Conclusion： FQ-PCR is a valuabl method for quantitative detection HPV 16/18DNA in cervical lesions.