摘要
采用氯胺-T法制备了^(125)I-HSA-IFN-α2b,标记率为82.7%,放化纯度>95%,放射性比活度为O.26 TBq/g。体外WISH/VSV系统抗病毒活性分析表明,^(125)I标记对HSA-IFN-β2b的生物学活性几乎无影响。^(125)I-HSA-IFN-α2b的血清和各组织的回收率分别为102.0%、104.0%(心脏)、100.4%(肺)、100.9% (肝)、104.1%(脾)、101.4%(肾)和103.0%(小肠),均符合方法学考核中回收率达90%～110%的要求。^(125)I-HSA-IFN-α2b的溶液干扰、血清样品干扰和组织样品干扰实验的结果表明,溶液、血清和组织样品对测定均无干扰,可以直接进行测定。根据实验结果可以初步推断,采用^(125)I-HSA-IFN-α2b同位素示踪法进行HSA-IFN-α2b的体内分布排泄研究是可行的。
125I-HSA-IFN-α2b was prepared with the method of CH-T. The labelling yields was 82. 72% ,radiochemical purity was more than 95% and the radioactivity was 0.26 TBq/ g. The activity of antivirus of 125I-HSA-IFN-α2b was compared with that of HSA-IFN-α2b by WISH/VSV system in vitro. The results showed that bioactivity of HSA- IFN-α2b had no change before and after being labelled. The serum recovery 102.0% and organ recovery 104.0% （heart）, 100. 4% （lung）, 100. 9% （liver）, 104. 1% （spleen）, 101. 4 % （kidney）, 103.0% （small intestine） of 125I-HSA- IFN-α2b met the requirements. The results of the interference experiments showed that there was no interference on the determination. Therefore, 125I-HSA-IFN-α2b can be used in studying biodistribution and excretion of HSA-IFN-α2b.
出处
《同位素》
CAS
北大核心
2008年第4期220-225,共6页
Journal of Isotopes
基金
卫生部科研基金资助项目(wkj2006-2-5)
江苏省人事厅"六大人才高峰"资助项目(06-B-026)
江苏省医学重点人才资助项目(RC2007097)
