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^(99)Tc^m间接标记NGR-IFN-α2a的方法研究 被引量：2

Study of Indirect ^(99)Tc^m Labelling NGR-IFN-α2a
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摘要 以双半胱氨酸(L,L-ethylenedicystein,EC)作为双功能螯合剂,MDP作为转移螯合剂,采用两步法对NGR-IFN-α2a进行^(99)Tc^m标记,制备了^(99)Tc^m-EC-NGR-IFN-α2a,并对标记和非标记的NGR-IFN-α2a进行活性鉴定。结果显示,EC-NGR-IFN-α2a合成产额为87.5%,^(99)Tc^m-EC-NGR-IFN-α2a标记率86%,放化纯度96%,在2 h内,标记物体外较稳定;EC-NGR-IFN-α2a与^(99)Tc^m-EC-NGR-IFN-α2a的活性与NGR-IFN-α2a无差异。 NGR-IFN-α2a was labelled with 99Tcm by two-step method used ethylenedicystein as a bifunctional chelating agent and MDP as a medium chelating agent. The bioactivities of EC-NGR-IFN-α2a and 99 Tcm_ EC-NGR-IFN-α2a were identified. It showed that the produc- tivity of EC NGR-IFN-α2a was 87.5%, the labelling yield rate and the radiochemical purity of 99Tcm- EC-NGR-IFN-α2a was 86% and 96%, respectively. In vitro, the radiolabelled compound had good stability,no bioactivity difference was found among EC-NGR-IFN-α2a, 99Tcm-NGR-EC-IFN-α2a and NGR-IFN-α2a.
作者 任炳秀 汪静 张路 王喆 杨卫东 张英起 李国权 贺丽清
机构地区 第四军医大学西京医院核医学科 第四军医大学生物技术中心
出处 《同位素》 CAS 北大核心 2008年第4期226-230,共5页 Journal of Isotopes
基金 国家自然科学基金资助项目(30570526)
关键词 99Tcm 间接标记 NGR 干扰素 99 Tcm indirect labelling NGR interferon
分类号 R817 [医药卫生—影像医学与核医学] R978.7 [医药卫生—药品]
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参考文献13

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共引文献9

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同位素
2008年 第4期

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