氧化苦参碱对青霉素致痫大鼠海马GABA、Glu及血清中细胞因子的影响

Influence of Oxymatrine on GABA,Glu in Hippocamps and Serum Cytokines of Penicillin Induced Epileptic Rats

目的研究氧化苦参碱(Oxymatrine,Oxy)对青霉素致痫大鼠海马内谷氨酸(Glu)、γ-氨基丁酸(GABA)和血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-2(IL-2)和白细胞介素-6(IL-6)含量的影响,探讨其抗癫痫的可能机制。方法以青霉素诱导SD大鼠癫痫模型,采用放射免疫测定法检测血清中TNF-α、IL-2和IL-6的含量,免疫组化SABC法测定海马谷氨酸(Glu)和γ-氨基丁酸(GABA)阳性神经元的含量。结果Oxy组与青霉素模型组比较:血清中TNF-α、IL-2和IL-6的含量明显降低(P<0.05),海马内GABA阳性细胞数明显增加,Glu阳性细胞数明显减少(P<0.01)。结论Oxy拮抗癫痫发作,机制可能与调节癫痫大鼠脑内的GABA与Glu的水平,降低TNF-α、IL-2和IL-6有关。

Objective To explore the effect of oxymatrine （Oxy） on the content of glutamate（Glu）, γ- amino butyric acid （GABA） in hippocampus and tumor necrotic factorsa （TNF-α）, interleukin-2 （IL-2）, interleukin-6 （IL-6） in serum of epileptic rats kindled by penicillin. Methods Penicillin kindled epileptic rat model was used. The level of TNF-α, IL-2, IL-6 in serum. Glu were determined by mdioimmunoassay and GABA immunoreactive positive cells were identified by SABC of immunohistochemical method. Results Level of TNF-α, IL-2 and IL-6 in serum in the Oxy group were significantly lower （ P 〈 0.05） than those in penicillin model group. GABA immunoreactive positive cells in the hippocamps were significantly increased. Glu immunoreactive positive ceils in the hippocamps were significantly reduced（ P 〈 0.01 ）in the Oxy group as compared with penicillin model group. Conclusion Oxy has the effect of antiepileptic activation. The mechanism of which may be related with the adjustment of the level of GABA, Glu and the level of cytokines and improvement of the imbalance of neuro-immuno-endocrine network.