大鼠肋膈肌和脚膈肌毛细血管密度观察

Observation of capillarity of rat costal and crural diaphragm

目的：观察和比较大鼠肋膈肌和脚膈肌毛细血管密度特征和差异。方法：采用肌球蛋白ＡＴＰ（ｍＡＴＰ）酶组化进行肌纤维分类和显示毛细血管，通过计算机图象分析系统，测定大鼠肋膈肌和脚膈肌毛细血管密度（ＣＤ）并与肢体肌进行比较。结果：①大鼠肋膈肌ＣＤ显著大于脚膈肌，两者ＣＤ均大于肢体肌肉；②不同类型肌纤维中，Ⅰ型和ⅡＡ型ＣＤ大于ⅡＢ；③同类纤维比较，肋膈肌ⅡＢ纤维ＣＤ大于脚膈肌和肢体肌，但Ⅰ和ⅡＡ型纤维各肌肉间无显著差异。结论：大鼠肋膈肌和脚膈肌毛细血管密度有显著差异，反应了两者功能要求不同，提示前者具有更强的氧化能力和耐力。

bjective: To observe the differences in capillary density (CD) of rat costal and crural diaphragm (DIcos and DIcru) and compare with two hindlimb muscles. Methods: The muscle fiber types and capillaries were identified by myosin ATPase histochemistry and the CD were measured by using a computerized imageprocessing system.Results:The CD of the DIcos is significantly higher than that of the DIcru and the two hindlimb muscles.The CD of type Ⅰand ⅡA is higher than type B.And a significant difference in CD of the type ⅡB fibers could be observed, which is higher in the DIcos than that of other three muscles.Conclusions:The present study showed that there are differences in CD between the DIcos and DIcru,which may be deter mined by their different functional demands.