胰腺癌细胞株SPARC基因启动子区甲基化状况的研究

SPARC gene expression in pancreatic cancer cell lines of its promoter methylation

目的检测6种胰腺癌细胞株中SPARC基因启动子区5’CpG岛甲基化状况。方法以ASPC、BX-PC3、CFPAC、PANC1、SW 1990、PaTu8988等胰腺癌细胞株及正常人胚肾细胞AD293标本为研究对象,采用甲基化特异性PCR(MSP)方法检测细胞株SPARC基因启动子区5’CpG岛甲基化状况。结果6例胰腺癌细胞株中3例(ASPC、CFPAC-1、BxPC3)细胞株发生完全甲基化;3例(PANC-1、Patu8988、SW 1990)发生部分甲基化,正常人胚肾上皮细胞AD293未发生甲基化。结论SPARC基因启动子区5’CpG岛甲基化改变是胰腺癌SPARC基因的一种重要失活方式,SPARC基因启动子区的高甲基化是胰腺癌细胞区别于正常细胞的分子事件之一,可能在胰腺癌的发生、发展中起重要作用。检测SPARC基因高甲基化可能会成为一种新的诊断胰腺癌的肿瘤标志物。

Objective To study the methylation status of SPARC gene in pancreatic cancer cell lines. Methods ASPC, Bx- PC3, CFPAC, PANC1, SW1990 and PaTu8988 in human pancreatic cancer cell lines were obtained in our laboratory. The HEKC AD293 of health adult was as controls. The change of the 5＇ CpG island methylation of SPARC gene was detected by methylation - specific PCR （MSP） in pancreatic cancer cell lines. Results There were three cases （ASPC, CFPAC - 1, BxPC3） had methylation among 6 pancreatic cancer cell lines （50%）. There were three cases （PANC - 1, Patu8988 and SW1990） had partly methylation among 6 pancreatic cancer cell lines （50%）. The HEKC AD293 of health adult didn＇t have methylatiou. Conclusion These results suggest that suppression of SPARC gene expression is associated with the 5＇CpG island methylation of the SPARC promoter region.