乙酰胆碱对大鼠齿状回成年神经发生的作用观察(英文)

Acetylcholine regulates adult neurogenesis in rat's DG

目的观察乙酰胆碱(ACh)对大鼠海马齿状回颗粒细胞下层成年神经发生的作用和影响。方法通过立体定位经侧脑室药物注射建立试验动物模型,实验组给予ACh,假手术组给予生理盐水,对照组只麻醉动物,不做任何手术处理,术后2h经腹腔给予核苷酸类似物BrdU。4周后处死动物,采用免疫组化的方法观察并计数海马齿状回颗粒细胞下层新生神经元。结果实验组海马齿状回BrdU+和BrdU+/CaBP+双标的细胞数目为637.00±39.50、491.00±47.29/hippocampi(N=3,n=36),而假手术组和对照组分别为339.00±17.62、305.00±17.62/hippocampi(N=3,n=36)和336.00±49.05、304.00±30.44/hippocampi(N=3,n=36),实验组分别与假手术组和对照组比较都有统计学差异(P<0.05),假手术组与对照组之间比较无统计学差异(P>0.05)。结论乙酰胆碱可以增加海马齿状回颗粒细胞下层新生神经元的数目,由此可能促进学习和记忆的形成。

Objective To observe the effects of acetylcholine（ACh）on adult neurogenesis in dentate gyrus（DG）of rats. Methods ACh was injected stereotaxically into the lateral ventricles of male rats in treated group, 0.9 % NaCl saline was injected in sham group, while the rats in control group were only anaesthetized. Immunohistochemistry was used to calculate the numbers of BrdU＋ and BrdU^＋/CaBP^＋ new born granule neurons in DG. Results The numbers of the BrdU^＋ and BrdU^＋/CaBP^＋ labeled cells in treated group were significantly higher（637.00 ±39.50 and 491.00 ± 47.29 per hippocampi, N = 3, n = 36） than in sham group （339.00± 17.62 and 305.00± 17.62 per hippocampi, N = 3, n = 36,P〈0.05）and in control group（336.00± 49.05 and 304.00± 30.44 per hippocampi, N = 3, n = 36, P 〈（0.05）, respectively, but there was no statistical difference between sham group and control group（P 〉0.05）. Comclusion These results indicate that ACh could promote the adult neurogenesis in DG and thus facilitate the learning and spatial memory.