替米沙坦对2型糖尿病大鼠肾组织MCP-1、NF-κB表达的影响

Effects of telmisantan on expression of monocyte chemoattractant protein-1,nuclear factor-kappa B in renal tissue of type 2 diabetic rats

目的观察替米沙坦对2型糖尿病大鼠肾脏病变中单核巨噬细胞趋化蛋白-1(MCP-1)、核因子-κB(NF-κB)表达的影响。方法雄性Wistar大鼠30只,随机分成正常对照组(A组,n=10)和糖尿病模型组(n=20)。A组给予常规饲料;模型组给予高糖高脂饲料,4周后腹腔注射链尿佐菌素(STZ)30mg/kg,造模成功后模型组再随机分成糖尿病组(B组,n=10)和替米沙坦治疗组(C组,n=10),C组每天给予替米沙坦5mg/kg灌胃。替米沙坦干预12周后,用免疫组化法检测各组大鼠MCP-1、NF-κB在肾脏表达的变化。结果与A组比较,B、C组大鼠肾组织中MCP-1、NF-κB表达明显增加(P<0.01);C组比B组MCP-1、NF-κB表达明显降低(P<0.01)。结论2型糖尿病大鼠肾组织中MCP-1、NF-κB表达明显增加,替米沙坦可通过降低肾组织MCP-1、NF-κB表达,从而减轻肾脏的炎症反应。

Objective To investigate the effects of telmisantan on expression of monocyte chemoattraetant protein-1 （MCP-1）and nuclear factor-kappa B（NF-κB）in renal tissues of type 2 diabetic rats. Methods Thirty Wistar rats were randomly divided into two groups： normal control group（ n = 10, group A）and diabetic group（ n = 20）. The rats were fed with standard diet in group A. The other rats were fed with high-glucose-high-fat diet. After 4 weeks, streptozotoein（30 mg/kg）was injected into abdominal cavity of the rats. Then the diabetic rats were randomly divided into 2 groups： model group（ n = 10, group B）and telmisanta group（ n = 10, group C）. The rats in group C were given telmisantan for 12 consecutive weeks. At the end of the 12th week, the expression of MCP-1 and NF-κB in kidney was measured by immunohistochemistry. Results The expression of MCP-1 and NF-κB in group B and group C was significantly higher than that in group A（P 〈 0.01）. The expression of MCP-1 and NF-κB in group C was lower than that in group B（P 〈 0.01 ）. Conclusion Telmisantan could decrease the renal inflammatory reaction by reducing the expression of MCP- 1 and NF-κB in kidney of diabetic rats, which might be the mechanism of renal protective effect of telmisantan.