可注射型及柱型藻酸钙载体联合运用修复兔膝关节软骨缺损的效果观察

Study on the effects of calcium alginate column scaffold combination with calcium alginate gel for repairing articular cartilage defects of rabbit's knee joint

目的:使用软骨细胞、可注射藻酸钙及柱型藻酸钙载体,修复兔股骨髁间非负重区骨软骨缺损,观察同种材料不同形态联合修复的效果。方法:将24只新西兰大白兔随机分成4组,第1组给予注射藻酸钙凝胶修复,第2组放置单纯柱型藻酸钙载体修复,第3组放置柱型藻酸钙载体后于周缘注射凝胶,第4组为空白不用任何支架材料修复。每只动物修复效果以Xij表示,以定量的方法统计出所有动物修复效果,以评分的形式表示,作统计学分析。结果:实验组与对照组一共4组数据,统计每组(i)中每只兔(j)膝关节软骨缺损修复效果评分Xij,然后进行组间比较。根据单向方差分析的思想,用SPSS12.0软件计算F=69.0,P<0.05,联合修复组效果好于其他各组。结论:柱型藻酸钙载体具有良好的成型效果且有一定的机械强度,可注射藻酸钙凝胶具有良好的黏附性,用于人工材料与正常结构的整合具有明显优势,两者联合修复软骨缺损,优势互补,符合软骨组织工程的修复与整合的要求。

Objective ：To repair cartilage defects at non-weight-bearing area of the femoral condyle in rabbits with invitro amplified cartilage cell using calcium alginate column scaffold combined with calcium alginate gel injection, and to study repair effects of combination with different form of the same material. Methods： Twenty-four New Zealand rabbits were divided into 4 groups randomly. The wounds of rabbits in the Group 1 were repaired with injection of calcium alginate gel ;the wounds of rab- bits in the Group 2 were repaired with in planting of calcium alginate column scaffold; the wounds of rabbits in the Group 3 were repaired with in planting of calcium alginate column supporter firstly, and then injection of calcium alginate gel at the surrounding;and Group 4 is control group, the rabbits in the group were repair and without any support. The repair effects were demonstrated with Xij, and the effects of all animals were studied with statistical analysis. Results：The Xij scores of each rabbits were calculated, and the scores in four groups were compared. The statistical results showed that combination therapy was better than other methods （F=69.0, P〈0.05 ）. Conclusion：The calcium alginate with column shape has better shaping effects and certain mechanical strength. The calcium alginate gel has better stick nature and can be used to integrate artifical material with normal structure. They can be used together, which meeting the desire of repair and integration in cartilaginous tissue engineering.