去卵巢骨质疏松模鼠DPD及1,25-(OH)_2D_3与骨质量的相关性研究

Study on the correlations between the bone quality and level of u-DPD and serum 1,25-(OH)_2D_3 in the ovariectomized osteoporosis rats

目的:探讨雌性SD大鼠切除卵巢后机体尿DPD及血1,25(OH)2D3变化与骨质量的相关性。方法:40只雌性SD大鼠随机分为卵巢切除组(OVX组)和假手术组(Sham组),分别于术后12、24周取材。运用双能X线骨密度仪测L1、股骨、股骨颈骨密度,ELISA法测尿DPD浓度,激素放免法测血1,25(OH)2D3浓度,光测弹性仪测L1压缩强度极限、弹性模量及右侧股骨弯曲破坏载荷,不脱钙骨切片技术观察胫骨上段骨组织形态结构并计量分析,对实验数据进行相关性分析。结果:术后OVX组除尿DPD/Cr明显升高外,其他检测指标均较Sham组低,且随术后时间推移持续下降,差异有统计学意义。相关分析显示:尿DPD/Cr与腰椎、股骨密度、椎体压缩强度极限、股骨弯曲破坏荷载和骨小梁面积均呈负相关。血清1,25(OH)2D3水平与腰椎、股骨、股骨颈密度、骨小梁面积、椎体压缩强度极限和股骨弯曲破坏荷载呈正相关。结论:大鼠卵巢切除后尿DPD、血1,25(OH)2D3水平变化与骨质量显著相关,可以通过检测尿DPD、血1,25(OH)2D3水平变化预测骨质量。

Objective：To study the correlations between the bone quality and level of u-DPD and serum 1,25-dihydroxyvitamin D3 in the ovariectomized osteoporosis rats. Methods：Forty female rats were randomly divided into the ovariectomy group（the OVX group） and the sham-operation group（the Sham group） ,and draw materials in 12 and 24 weeks after operation. Bone mineral density （BMD） of the I st lumbar, the femoral neck and femur was measured by dual-energy X-ray absorptionetry. The level of urine DPD was measured by ELISA. The concentration of 1,25-dihydroxyvitamin D3 in serum was measured by radioimmunoassay （RIA）. The limit of compressive strength and elastic modulus of 1st lumbar and bending toad of right femur were measured by photoelasticity meter. The osseous morphology of upper tibia was measured by the non-decalcifing section. The correlation of experimental data was analyzed by statistical method. Results： In ovariectomy group, except the level of urine DPD/Cr was increase, all data decreased significantly compared with the samp group, and descending continually with the lapse of time. The results showed that there was a negative correlation between the urine DPD/Cr and BMD of the 1st lumbar, femur, the limit of compressive strength of 1st lumbar, bending load of femur and trabeeular bone area. But there was a positive correlation significantly between the concentration of 1,25-dihydroxyvitamin D3 in serum and BMD of the 1st lumbar,the femoral neck and femur,trabecular bone area,the limit of compressive strength of 1st lumbar, bend load of femur. Conclusion： The correlation is remarkable between the bone quality and the level of urine DPD/Cr, 1,25-dihydroxyvitamin D3 in serum. We can predict the bone quality by measuring the level of urine DPD/Cr and 1,25-dihydroxyvitamin D3 in serum.