参附通过抗凋亡途径对大鼠肝脏缺血再灌注的影响

EFFECT OF SHENFU INJECTION ON HEPATIC APOPTOSIS DURING ISCHEMIA-REPERFUSION IN RATS

目的:研究参附注射液对大鼠肝缺血再灌注期间肝细胞凋亡的影响,并探讨其机制。方法:采用Pringle’s法建立大鼠肝缺血再灌注模型。54只Wistar大鼠随机分为假手术组(S组)、缺血再灌注组(IR组)和参附治疗组(SF组)。在规定时间检测肝细胞Bcl-2、Caspase-3蛋白的表达、细胞凋亡,同时观察病理形态学改变。结果:细胞凋亡指数在各时间点IR组显著增多,SF组较IR组显著减少而高于S组。与IR组比较,SF组的Bcl-2蛋白表达在再灌注6h、24h提高的显著;而Caspase-3蛋白表达在再灌注2h、6h下降的显著。结论:参附注射液通过抗细胞凋亡途径保护肝脏缺血再灌注损伤。SF通过下调Caspase-3表达,同时上调Bcl-2表达而抑制再灌注时肝细胞凋亡,减轻肝缺血再灌注损伤。

Objective：To investigate effects of Shenfu injection on apoptosis of hepatic cells during ischemia-reperfusion in rats and to discuss the mechanism. Methods：Hepatic ischemia -reperfusion injury（HIRI） model was established by Priagle＇ s method. Fifty-four health Wistar rats were randomized into sham - operated group （ Group S, n = 18 ）, ischemia reperfusion group （ Group IR, n = 18 ） and Shenfu preconditioning group （ Group SF, n = 18 ）. The sample of liver tissue taken from all rats for determination of hepatocel-lular apoptosis, expression of Bcl -2 and Caspase -3 and liver morphological changes. Results：The apoptosis index （AI）of liver tissue in Group SF were lower than in Group IR significantly 2,6 and 24 hours after reperfusion. Compared with Group IR, the expression of Bcl - 2 was enhanced significantly in Group IR 6 and 24 hours after reperfusion and the expression of Caspase - 3 was reduced signifi- cantly in Group IR 2 and 6 hours after reperfusion. Conclusion ： Shenfu precondioning pretects hepatic ischemia - reperfusion injury effectively in ral via an antiapoptotic pathway. The antiapoptotic mechanism of Shenfu may include inhibiting the expression of Caspase - 3 and enhancing the expression of Bcl -2.