紫外线辐射灭活斑马鱼精子及其激活卵子发育的初步研究

Priliminary Studies on UV Irradiation for the Inactivation of Sperm and Development of Fertilized Oocyte in Zebrafish

精子的灭活是雌核发育过程中的一个关键步骤,为了研究不同介质中斑马鱼精子的灭活效果,将斑马鱼的精子分别置于纯水(A)、Cortland鱼用生理盐水(B)、M199培养基(C)、Hank's液(D)以及Ringer氏溶液(E)中进行紫外线照射,然后与正常卵子进行人工受精,统计受精率、孵化率以及畸形率。结果显示,各种介质中均得到畸形鱼苗,并有明显的单倍体综合症现象;五种介质中在Cortland鱼用生理盐水中平均受精率最高;综合受精率和畸形苗的比例,35s辐射比较适于斑马鱼精子灭活与激活卵的发育。畸形鱼苗的染色体检测结果显示,其染色体众数为25,为斑马鱼染色体数目的一半,少数分裂相的数目介于25和50之间。

The genetic inactivation of sperm is the first key stage of gynogenesis in fish. In order to study the effect of UV irradiation for the inactivation of sperm of zebrafish, the sperm were irradiated by UV with solution： pure water（A）, Cortland solution（B）, medium M199（C）, Hank＇s solution（D） and Ringer＇s solution（E）. And then, we mixed the irradiated sperm and the normal eggs in relevant solution, and counted the fertilization rate, incubation rate and abnormality rate. The results showed that all of the five groups obtained abnormal fry, and the phenomenon of ＂haploid syndrome＂ were validated. The average of fertilization rate in the Cortland solution was better than that of the rest four groups. Compared fertilization rate with abnormality rate, 35 seconds of irradiation time can inactive fully. The results of the test of chromosome indicated that chromosome number of most of abnormal fish was 25, and few of them between 25 and 50.