糖尿病大鼠血管内皮细胞DNA损伤与凋亡的研究

Study on DNA damage and cell apoptosis in the blood vessel endothelial cell of diabetic rats

目的探讨糖尿病（diabetes mellitus）大鼠血管内皮细胞DNA损伤与凋亡的关系。方法制作链脲佐菌素（STZ）诱导的糖尿病模型大鼠。16只SD雄性大鼠随机分为正常对照组、糖尿病大鼠组，分离血管内皮细胞。采用单细胞琼脂糖凝胶电泳检测细胞DNA损伤，采用流式细胞仪分析检测凋亡。结果糖尿病组与对照组血管内皮细胞DNA损伤积分分别为（29．5±6．7）分和（8．6±3．6）分，细胞凋亡率为（10．9±3．5）和（4．4±1．2）。2组之间相比有差异有统计学意义（P〈0．05）。将细胞凋亡率与DNA损伤程度进行了相关分析，发现两者之间呈明显的正相关（r=0．82，P〈0．05）。结论糖尿病大鼠血管内皮细胞有明显的DNA损伤和凋亡，且细胞凋亡与DNA损伤明显相关，但血管内皮细胞DNA损伤和凋亡在糖尿病中的作用尚需进一步的研究。

Objective To explore the relationship of DNA damage and cell apoptosis in the blood vessel endothelial cell of diabetic rats. Methods Strcptozotoein was used to make diabetic rats, 16 rats were random divided into diabetic group and control group. DNA damage was measured by comet assay and cell apoptosis was detected by flow cytometry. Results DNA damage score was 29. 5 ± 6. 7 and 8.6 ±3.6 in diabetic rats and controls, the rate of cell apoptosis was respectively 10. 9±3.5 and 4.4 ± 1.2. It had a significant difference between two groups in DNA damage and cell apoptosis（ P 〈 0. 05 ）. The rat of cell apoptosis had strongly positive correlation with DNA damage scores （ r = 0. 82, P 〈 0. 05 ）. Conclusion DNA damage and cell apoptosis are found in diabetes mellitus, cell apoptosis may be associated with DNA damage. The roles of DNA damage and cell apoptosis in diabetes mellitus need further research.