苦参碱诱导人喉癌细胞Hep-2凋亡的实验研究

Apoptosis of human laryngeal carcinoma Hep-2 cell induced by matrine

目的研究苦参碱对人喉癌细胞Hep-2的生长抑制作用及凋亡机制。方法四甲基噻唑蓝（MTT）法测定苦参碱对细胞的抑制作用，荧光显微镜、DNA琼脂糖凝胶电泳、流式细胞仪技术（FCM）观察细胞周期及凋亡。结果不同浓度苦参碱对Hep-2细胞有明显抑制作用，且呈时间依赖性及剂量依赖性；FCM检测结果显示：S期细胞比例及凋亡率增高，G2／M期细胞比例下降，且呈剂量依赖性；形态学发现Hep-2细胞染色质凝聚，核碎裂，凋亡小体形成；DNA琼脂糖凝胶电泳显示典型的梯形条带。结论苦参碱可能是通过将人喉癌细胞周期阻滞在S期，诱导喉癌细胞凋亡，抑制其增殖，发挥其抗癌功能。

Objective To investigate the mechanism of grow-inhibition and apoptosis induced by marine on Hep-2 line. Methods The growth inhibiting rate of matrine on Hep-2 cells was detected by MTr assay. Fluorescence microscope, DNA gel eleetrophoresis and flow eytometry （FCM） were applied to determine the presence of apoptosis and cell cycle. Results Matrine had cytotoxie effect to Hep-2 cells in a time- and dose-dependent manner. The FCM analysis showed that the number of Hep-2 cells of S phase and the apoptosis rate increased, while the number of G2/M phase decreased. There were morphological changes including ehromatin condensation, nuclear fragmentation and apoptotic bodies formed. DNA gel electrophoresis analysis showed typical DNA ladder of apoptosis. Conclusion Matrine inhibits cell proliferation by blocking Hep-2 cell cycle to S phase, and exerts its anti-carcinoma function by inducing apoptosis.