基于线粒体基因Cytb和COI的蝗科中五亚科分子系统发育关系分析(直翅目:蝗总科)(英文)

Molecular phylogenetic analysis of five subfamilies of the Acrididae (Orthoptera:Acridoidea) based on the mitochondrial cytochrome b and cytochrome c oxidase subunit I gene sequences

本研究基于Cytb基因和COI基因的部分序列来推断17种蝗虫之间的系统发育关系。这17种蝗虫均采自国内,代表了蝗科(Acrididae)5个亚科:黑蝗亚科(Melanoplinae)、斑腿蝗亚科(Catantopinae)、刺胸蝗亚科(Cyrtacanthacridinae)、斑翅蝗亚科(Oedipodinae)和大足蝗亚科(Gomphocerinae)。采用联合序列方法进行分析,结果显示:Cytb和COI联合序列长度为1998bp,其中A和T总含量为72.13%,G和C总含量为27.87%。联合序列共包含了889个保守位点,1109个变异位点,在这些变异位点中有838个简约信息位点。系统发生树采用邻接法(NJ)、最大简约法(MP)和最大似然法(ML)进行构建。使用蜢总科的变色乌蜢Erianthus versicolor和Erianthus sp.两个种作为外群。结果表明:大足蝗亚科和斑腿蝗亚科的单系性没有得到支持。斑翅蝗亚科内部各种聚成一个大支,在本研究中该亚科的单系性得到支持,与前人的研究结论相同。大足蝗亚科、斑腿蝗亚科、刺胸蝗亚科和黑蝗亚科这4科关系非常近,可以考虑将其合并为一个亚科。同时,我们发现基于Cytb和COI基因联合序列推断蝗科内各亚科间的系统发生关系并不十分可靠。

The homologus sequences of the mitochondrial cytochrome b （ Cytb ） and cytochrome c oxidase subunit I （COI） genes were sequenced in 17 species representing five subfamilies （belonging to family Acrididae）： Melanoplinae, Catantopinae, Cyrtacanthacridinae, Oedipodinae and Gomphocerinae from China. The concatenated sequence from both genes was 1 998 bp in length, consisting of 1 266 bp and 732 bp for COI and Cytb respectively. In the concatenated sequence, A ＋ T content was about 72.13%, and C ＋ G only 27.87%. There are 889 sites conserved, 1 109 sites were variable, and 838 sites were parsimony-informative in the variable sites. Phylogenetic trees were reconstructed with maximum parsimony, neighbor-joining, and maximum likelihood using Erianthus versicolor and Erianthus sp. of the Eumastacoidea as outgroups. Our results showed that the monophyly of the subfamilies Gomphocerinae and Catantopinae were not supported here. The phylogenetie trees did not entirely agree with the international taxonomic system of grasshoppers. The subfamily Oedipodinae clustered as one clade, which was supported as a monophyletic group. Based on the close relationships among four subfamilies, Gomphocerinae, Catantopinae, Cyrtacanthacridinae and Melanoplinae, we suggest that the four subfamilies should be considered to merge into one subfamily. Simuhaneously, we found it was not very dependable to infer the phylogenetic relationships among subfamilies within the Acrididae based on the concatenated sequence from Cytb and COI genes.