期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

胰岛素样生长因子-Ⅱ对妊娠高血压综合征胎盘滋养细胞浸润能力的影响 被引量：4

Effect of insulin-like growt factor-Ⅱ to invasion of placental trophoblast in PIH
下载PDF
导出
摘要 目的测定胎盘滋养细胞中胰岛素样生长因子一Ⅱ(insulin-like growth factor-Ⅱ,IGF-Ⅱ)的表达,评价在妊娠高血压综合征(妊高征)中IGF-Ⅱ对胎盘滋养细胞浸润能力的影响。方法用免疫组化SP法,测定36例正常孕妇、34例妊高征孕妇胎盘组织中IGF-Ⅱ蛋白的表达,并用计算机图像分析系统进行定量分析比较。结果①在两组孕妇的胎盘组织中,其合体滋养细胞、细胞滋养细胞、羊膜绒毛层均能检测到IGF-Ⅱ蛋白的表达,但羊膜绒毛层染色与上述两种细胞相比明显减弱。②妊高征组胎盘中的IGF-Ⅱ与正常组相比,平均光密度显著降低(t=2.528,P<0.05)。③轻、中、重度妊高征胎盘滋养细胞中,IGF-Ⅱ的平均光密度呈现逐渐下降的趋势,但三者之间的差异未见明显统计学意义(P>0.05)。结论在妊娠高血压综合征中,胎盘中IGF-Ⅱ的表达降低可能对滋养细胞的浸润能力产生重要影响。 Objective To investigate the expression of insulin-like growth factor- Ⅱ （IGF- Ⅱ ） in human placenta and to evaluate effect of insulin-like growth factor- Ⅱ on the invasion of placental trophoblast in pregnancy induced hypertension syndrome（PIH）. Methods SP immunohistochemical staining method was used to detect the protein of IGF- Ⅱ in placenta of 36 normal cases and 34 PIH cases. The mean density was determined by image analysis technique. Results （1） The positive staining of IGF-Ⅱ was found in the syncytiotrophoblast, cytotrophoblast, amnio-chorion of both normal cases and PIH cases, but less immunoreactivity in the amnio-chorion. （2） The expression level of IGF- Ⅱ in PIH placenta was significantly lower than that of the control group（ t = 2. 528, P〈0.05）. （3） From mild to severe PIH, the mean density of IGF-Ⅱ decreased gradually,but had no statistical difference （P 〉0.05）. Conclusions The lower expression of IGF-Ⅱ may play an important role in the invasion of placental trophoblast in PIH.
作者 邹梅芳 陈燕 王海燕
机构地区 江苏省常州市武进中医医院
出处 《实用临床医药杂志》 CAS 2008年第7期29-31,共3页 Journal of Clinical Medicine in Practice
关键词 胰岛素样生长因子-Ⅱ 妊娠高血压综合征 滋养细胞 insulin-like growt factor- Ⅱ pregnancy induced hypertension syndrome trophoblast
分类号 R714.24 [医药卫生—妇产科学]
  • 相关文献

参考文献12

  • 1Fisher SJ, Damsky CH. Human cytotrophhlast invasion[J ]. Seminars in cell biology, 1993, 4: 183.
  • 2Chakraborty C, Glesson M, McKirmon T, et al. Regulatiob of human trophoblast migration and invasiveness[J ]. Can J P hysiol Pharmacol, 2002, 80(2): 116.
  • 3Bayes-Genis A, Conover C A, Schwartz R S. The Insulin-Like Growth Factor Axis, A Review of Atherosclerosis and Restenosis[J]. Circulation Research, 2000, 86: 125.
  • 4Qingquan Liu, Huajun Yan, Dawes N J, et al. Insulin-like Growth Factor Ⅰ Induces DNA Synthesis in Fetal Ventricular Myocytes In Vitro [J]. Circulation Research, 1996, 79: 716.
  • 5Liu H C, He Z Y, Mele C A, et al. Expression of IGFs and their receptors is a potential markers for embryo quality[J ]. Am Reprod Immunol, 1997, 38: 237.
  • 6Const ncia M, Hemberger M, Hughes J, et al. Placentalspecific IGF-Ⅱ is a major modulator of placental and fetal growth[J]. Nature, 2002, 417(6892): 945.
  • 7Staun-Ram E, Goldman S, Gabarin D, et al. Expression and importance of matrix metalloproteinase 2 and 9 (MMP-2 and -9) in human trophoblast invasion [J]. Reprod Biol Endocrinol, 2004, 2: 59.
  • 8Irwin J C,Suen L F, Faessen G H, et al. Insulin-like growth factor (IGF)-II inhibition of endometrial stromal cell tissue inhibitor of metalloproteinase-3 and IGF-binding protein-1 suggests paracrine interactions at the decidua: trophohlast interface during human implantation [ J ]. J Clin Endocrinol Metah, 2001, 86(5): 2060.
  • 9Hills F A, Elder M G, Chard T, et al. Regulation of human villous trophoblast by insulin-like growth factors and insulinlike growth factor-binding protein-l[J]. J Endocrinol, 2004, 183(3) : 487.
  • 10Shin J C, Lee J H, Yang D E, et al. Expression of insulinlike growth factor-Ⅱ and insulin-like growth factor binding protein-1 in the placental basal plate from pre-eclamptic pregnancies [J]. Int J Gynaecol Obstet, 2003, 81(3): 273.

二级参考文献9

  • 1乐杰.妇产科学[M](第5版)[M].北京:人民卫生出版社,2001.259.
  • 2Cross J C, Hemberger M, Lu Y, et al. Trophoblast functions, angiogenesis, and remodeling of the maternal vasculature in the placenta[J]. Mol Cel Endocrinol, 2002, 187(1-2): 207-212.
  • 3Matijevic R, Johnston T. In vivo assessment of failed trophoblastic invasion of the spiral arteries in pre-eclampsia[J]. Br J Obstet Gynecol, 1999, 106(1): 78-82.
  • 4Chakraborty C, Gleeson L M, McKinnon T, et al. Regulation of human trophoblast migration and invasiveness[J]. Can J Physiol Pharmacol, 2002,80(2): 116- 124.
  • 5hin J C, Lee J H, Yang D E, et al. Expression of insulin-like growth factor- Ⅱ and insulin-like growth factor binding protein-1 in the placental basal plate from pre-eclamptic pregnancies[J]. Int J Gynaecol Obstet, 2003, 81(2): 273 - 280.
  • 6Constancia M, Hemberger M, Hughes J, et al. Placental-specific IGF- Ⅱ is a major modulator of placental and fetal growth[ J ]. Nature, 2002, 417(6892): 945 - 948.
  • 7Giudice L C, Conover C A, Bale L, et al. Identification and regulation of the IGFBP-4 protease and its physiological inhibitor in human trophoblasts and endometrial stroma: evidence for paracrine regulation of IGF-Ⅱ bioavailability in the placental bed during human implantation[J]. J Clin Endocrinol Metab, 2002, 87(6): 2359-2366.
  • 8Irwin J C, Suen L F, Faessen G H, et al. Insulin-like growth factor(IGF)-Ⅱ inhibition of endometrial stromal cell tissue inhibitor of metalloproteinase-3 and IGF-binding protein-1 suggests paracrine interactions at the deciduas:trophoblast interface during human implantation [ J]. J Clin Endocrinol Metab, 2001, 86(5): 2060-2064.
  • 9Aplin J D, Lacey H, Haigh T, et al. Growth factor-extracellular matrix synergy in the control of trophoblast invasion [ J ]. Biochem Soc Trans,2000, 28(2): 199 - 202.

共引文献1

同被引文献19

引证文献4

二级引证文献4

实用临床医药杂志
2008年 第7期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部