氯胺酮对脂多糖诱导的大鼠肺泡巨噬细胞氧化应激的影响

Effect of ketamine on oxidative stress in Lipopolysaccharide-stimulated rat alveolar macrophage cell line

目的研究氯胺酮对脂多糖(LPS)刺激的大鼠肺泡巨噬细胞氧化应激的影响。方法体外培养的大鼠肺泡巨噬细胞株NR8383经氯胺酮(10、100和1000μmol/L)预处理,LPS刺激24 h后,应用试剂盒检测细胞培养上清液中丙二醛(MDA)及超氧化物岐化酶(SOD)的水平。结果与PBS组比较,LPS组培养上清液中MDA水平均明显增加,而SOD水平显著下降,而氯胺酮预处理组(100和1000μmol/L)对此具有抑制作用。结论氯胺酮对LPS所致的NR8383细胞的氧化应激损伤具有一定的保护作用。

Objective To investigate the effect of ketamine on oxidative stress in the supernatant in the rat alveolar macrophage cell line （NR8383） activated by Lipopolysaceharide （LPS）. Methods Macrophage was pretreated with ketamine at concentration of 10, 100 and 1 000 μmol/ L, then was stimulated with LPS. The production of SOD and MDA in the supernatant of macropahge was assayed by SOD and MDA kit. Results LPS induced an increase of MDA and an decrease of SOD in the supernatant. Pretreatment with ketamine（100 and 1 000/μmol/L） can in- hibited this response. Conclusion Ketamine exerted a protective effect on LPS- induced oxidative stress injury of NR8383.