摘要
研究了黑曲霉液体发酵产聚半乳糖醛酸酶的培养基优化.研究表明,最佳发酵培养基为(g/L):麸皮40,鲜苹果渣20,(NH4)2SO420,NaCl1,MgSO4·7H2O6,KH2PO41.最佳初始pH3.5,接种量3%,装液量50,mL,发酵时间3,d.经过培养基优化,酶活可达到1,505.8,U.用其处理BCTMP(化学热磨机械浆)的DCS(溶解物质和胶体物质)水,处理30,min后,DCS水的CD(阳电荷需求量)降低27%.
The optimal medium for polygalacturonase were studied. The results of the experiment show that the optimal medium are （ g/L ） ： wheat bran 40, fresh apple residue 20, （ NH4 ）2SO4 20, NaCl 1, MgSO4·7H2O6, KH2PO4 1. Other fermentation conditions are： pH 3.5, inoculation amount 3%, liquid volume flask 50 mL and fermentation time 3 days. Under these conditions, the enzyme activity of polygalacturonase reaches 1 505.8 U. Using the polygalacturonase to treat DCS water of BCTMP, the CD value of DCS water decrease 27% after reacting 30 min.
出处
《天津科技大学学报》
CAS
2008年第4期1-5,共5页
Journal of Tianjin University of Science & Technology
基金
国家自然科学基金资助项目(30771694)
天津市科技发展计划项目(06TXTJJC14302)
关键词
黑曲霉
聚半乳糖醛酸酶
液体发酵
DCS
Aspergillus niger
polygalacturonase
liquid-state fermentation
DCS
