牛血清白蛋白与三唑酮结合作用的热力学研究

Thermodynamic Studies on the Binding Interaction Between Tradimefon and Bovine Serum Albumin

在模拟人体生理条件下,采用分子光谱法研究了三唑酮与牛血清白蛋白的相互作用。结果发现,三唑酮对牛血清白蛋白的内源荧光有一定的猝灭作用,由Stern-Volmer方程和Vant′Hoff等压方程分析处理实验数据,得到了结合作用的平衡常数、结合反应的热力学参数、结合作用力类型等。

The interaction of tradimefon to bovine serum albumin （BSA） was studied using molecular spectroscopy by imitating human body physiology environment. The result showed that this compound had a powerful ability to quench the BSA fluorescence. By analyzing the spectral data with Stern-Volmer equation and Van＇t Hoff equation, the static fluorescence quenching binding constant, the thermodynamic parameters, and the binding power were obtained.