鸡腿蘑发酵液中抑制非酶糖糖基化反应活性物质的分离及分析

Separation of a Bioactive Substance Inhibiting Non-enzymatic Glycation from Coprinus comatus Fermentation Broth

以体外非酶糖基化抑制率为主要检测指标,对鸡腿蘑发酵液中抑制非酶糖基化反应具有较高活性的物质进行了初步的分析与分离,经过AB-8大孔树脂和C18反相柱的多次分离,获得一种具有高非酶糖基化反应抑制活性物质鸡腿蘑素(Comatin),经HPLC-MS分析其相对分子质量为168。在2 mg/mL质量浓度条件下,体外非酶糖基化抑制率达到95.86%,半抑制质量浓度IC50为0.39 mg/mL,体外实验初步分析鸡腿蘑素可能与蛋白质某一位点结合,且这种结合是在己二醛与牛血清白蛋白结合之后。

Comatin, exhibit high inhibition percentage on the non-enzymatic glycation （NEG） reaction, was obtained from the Coprinus comatus fermentation broth, and absorbed by macropopous resin and then separated with C18 reversed-phase chromatography. Its molecular weight was 168 determined by HPLC-MS. Furthermore, in vitro experiment showed that the IC50 of Comatin on inhibition the NEG reaction was 0. 39 mg/mL, and also found that Comatin could combine with Amadori products to change their structure and finally attenuate their fluorescence intensity.