丹参酚酸B的代谢产物研究

Studies on the Metabolites of Salvianolic Acid B

目的:分离鉴定大鼠灌胃丹参酚酸B后尿液中的代谢产物。方法:大鼠尿液的醋酸乙酯萃取物用聚酰胺柱色谱和制备HPLC方法分离纯化;根据谱学数据进行结构鉴定。结果:从大鼠尿液醋酸乙酯萃取物中分离鉴定了5个化合物,分别是8-羟基-3,4-二氢喹啉-2(1H)-酮(1),1-甲基-1H-吲哚-2-羧酸(2),反式-3-(3-羟基苯基)丙烯酸(3),1H-吲哚-2-羧酸(4),8-氨基喹啉-2(1H)-酮(5)。结论:化合物1,2,4和5为首次从灌胃丹参酚酸B大鼠尿液中分离得到。

Objective： To study the metabolites of EtOAc layer of the rat urine which was collected after after oral administration of Sal B. Methods： The constituents were isolated by chromatography methods of polyamide and PHPLC. The structure of these compounds were identified by NMR spectroscopic methods. Results： Compounds 1-5 were obtained and their structures were identified as 8-hydroxy-3, 4-dihydroquinolin-2 （1 H） -one（ 1 ）, 1-methyl-1 H- indole-2-carboxylic acid （ 2 ）, （E） -3- （3-hydrox-ylphenyl） acrylic acid （ 3 ）, 1 H-indole-2-carboxylic acid （ 4 ）, 8-aminoquinolin-2 （ 1 H） -one （5） . Conclusion： Compound 1,2, 4, 5 are isolated from urine of rats after oral administration of Sal B for the first time.