子宫内膜异位症患者腹腔液和血清IL-6、IL-15的临床分析

The clinical analysis of IL-6,IL-15 in peritoneal fluid and serum in patients with endometriosis

目的探讨腹腔液和血清白细胞介素（IL）-6、IL-15与子宫内膜异位症（EM）的关系。方法EM组为EM患者74例（其中Ⅰ～Ⅱ期26例，Ⅲ～Ⅳ期48例），对照组为卵巢良性畸胎瘤或行输卵管结扎术患者46例，采用双抗体夹心ELISA法测定两组患者腹腔液和血清IL-6、IL-15的水平。结果EM组腹腔液和血清IL-6[（1017．81±361．98）ng/L和（455．47±161．52）ng／L]均高于对照组[（284．63±70．50）ng/L和（149．37±43．09）ng／L]，EM组Ⅲ～Ⅳ期腹腔液和血清IL-6[（1253．44±189．63）ng/L和（556．504-93．34）ng/L]较Ⅰ～Ⅱ期[（582．81±107．75）ng/L和（268．964-63．48）ng/L]升高，差异均有统计学意义（P〈0．01），腹腔液和血清IL-6呈正相关（r=0．950，P=0．01）。EM组腹腔液IL-15[（333．45±63．94）ng／L]较对照组[（203．85±70．52）ng/L]升高，差异有统计学意义（P〈0．01），EM组和对照组血清IL-15比较差异无统计学意义（P〉0．05）；EM组Ⅲ-Ⅳ期腹腔液和血清IL-15与Ⅰ～Ⅱ期比较，差异均无统计学意义（P〉0．05）。结论腹腔液IL-6、IL-15的改变可能与EM的发病机制有关，血清IL-6可能成为诊断EM并评估其分期的无创性指标之一。

Objective To investigate the relationship between the levels of intedeukin（IL）-6, IL-15 and endometfiosis （EM）. Method The levels of IL-6, IL-15 in the peritoneal fluid （PF） and serum of 74 patients with EM （EM group） and 46 patients without EM （control group） were measured by double-antibodyELISA. Results Higher levels of IL-6 in PF and serum were observed in EM group [（1017.81± 361.98） ng/L,（455.47 ± 161.52） ng/L] than those in control group [（284.63±70.50） ng/L,（149.37 ±43.09） ng/L], and there was significant difference （P〈O.01）. The levels of IL-6 in PF and serum in EM group with stage Ⅲ-Ⅳ [ （1253.44± 189.63） ng/L,（556.50±93.34） ng/L] were significantly higher than those in patients with stage Ⅰ - Ⅱ[ （582.81 ± 107.75 ） ng/L, （268.96 ± 63.48 ） ng/L ] （P 〈 0.01 ）. There was positive relationship between the levels of IL-6 in PF and serum （r = 0.950, P= 0.01 ）. The levels of IL-15 in PF in EM group [ （333.45 ±63.94） ng/L]were significantly higher than those in control group[ （203.85 ± 70.52） ng/L] （P 〈 0.01 ）. No significant difference was found in the levels of serum IL-15 between EM group and control group （P〉 0.05）. No significant difference was observed either in the levels of IL-15 in PF and serum between patients with stage Ⅲ-Ⅳ and stage of EM Ⅰ-Ⅱ （P〉0.05）. Conclusions The increase of IL-6, IL-15 in PF may contribute to the development of EM. Serum IL-6 levels are of clinical diagnostic value in patients with EM.