黄芩中天然防晒剂的索氏提取工艺研究

Research on the Extraction of Natural Sunscreen from the Radix Scutellariae by Soxhlet Extraction

以确定黄芩药材中总黄酮类化合物提取的最佳提取条件为目的。以黄芩总黄酮得率为参考指标,采用单因素试验和正交设计法对提取时间（4、5、6 h）、溶剂中乙醇用量（60、80、100mL）以及物料粒径数（10-40、40-70、70目）等影响因素进行考察,确定最优提取条件。结果表明：提取黄芩药材中黄酮类成分的最佳提取方案为乙醇用量100 mL,提取6 h,物料粒径数为40-70目。通过方差分析发现3个因素对黄芩的提取影响都不显著。乙醇索氏提取法简便、省时、经济,在测定总黄酮时可以考虑用乙醇索氏法来提取。试验为防晒霜的配置提供了一定的试验基础。

To ascertain the optimized condition for extraction of flavones from Radix Scutellariae. The contents of flavonoids were taken as the indices and were determined. The extracting time（4 h, 5 h ,6 h）,volume of ethanol （60 mL, 80mL, 100 mL） and the size of materials（10-40,40-70,70）were analyzed by orthogonal design and univariate;The optimized condition were as follows：extraction time was 6 h, size of materials was 40-70,extraction volume was 100 mL. Ethanol Soxhlet extraction method was simple, effective and practical, and can be used to determine the total flavones from Radix Scutellariae when necessary.