类人胶原蛋白基因工程菌生长期动力学模型

Kinetics model of recombinant Escherichia coli BL21 expressing human-like collagen in growth phase

研究类人胶原蛋白基因工程菌生长期动力学模型,为优化发酵调控提供理论依据和指导。应用元素衡算和代谢衡算(EMB)方法,建立类人胶原蛋白基因工程菌生长期的动力学模型,并利用线性规划法对动力学模型参数αh和mX进行估算。结果表明,αh和mX分别为1.173 mol/C-mol和2.326 mol/(C-mol.h),该模型能较好地预测重组大肠杆菌生长期的宏观反应速率,且确立了葡萄糖的流加方程。利用EMB方法建立的动力学模型具有重要的应用价值,若要进一步减小氮消耗速率和CO2产生速率预测误差,需要对重组大肠杆菌的代谢网络进行系统分析,建立该模型的修正系数。

In order to optimize fermentation control, the kinetics model of recombinant Escherichia coli BL21 expressing the human-like collagen was investigated. On the basis of element and metabolism balancing （EMB）, the kinetics model of recombinant Escherichia coli BL21 during growth phase was developed, and the parameters of ah and mx in the model were estimated by the method of linear optimization. The parameters of ah, and mx are 1. 173 mol/C-mol and 2. 326 mol/（ C-mol · h） respectively. This model could preferably predict the macroscopic reaction rates, and the glucose-feeding formula was ascertained. The kinetics model based on EMB is very useful for application. If it is necessary to decrease the predication error of nitrogen consumption rate and CO2 production rate further, the metabolic net of recombinant Escherichia coli BL21 should be analyzed systemically to establish the amendment coefficients of the kinetics model.