O^6-甲基鸟嘌呤-DNA甲基转移酶基因多态性与新疆哈萨克族食管癌易感性关系的研究

Study on Relationship Between Polymorphisms of O^6-methylguanine-DNA Methyltransferase Gene and Susceptibility of Esophageal Cancer in Kazak Population

目的为探讨O6-甲基鸟嘌呤-DNA甲基转移酶(MGMT)基因多态性与新疆哈萨克族食管癌易感性的关系。方法运用病例-对照研究的方法,PCR-RFLP技术检测新疆哈萨克族食管癌51例及非癌对照109例的MGMT84C>T和MGMT135G>T单核苷酸多态(SNPs)的基因型。结果MGMT84C>T位点的三种基因型CC,CT,TT,在哈萨克族食管癌中所占比例分别为76.5%(39/51)、17.6%(9/51)、5.9%(3/51),对照组分别为77.0%(84/109)、18.3%(20/109)、4.6%(5/109),两组间分布差异不显著(P=0.938,P>0.05);MGMT135G>T位点的三种基因型GG,GT,TT,在哈萨克族食管癌中所占比例分别为90.2%(46/51)、7.8%(4/51)、2.0%(1/51),对照组分别为82.6%(90/109)、16.5%(18/109)、0.9%(1/109),两组间分布差异不显著(P=0.295,P>0.05);MGMT84C>T位点和MGMT135G>T位点联合分析,在食管癌组0突变等位基因与1-4突变等位基因所占比例分别为68.6%(35/51)、31.4%(16/51);对照组分别为:63.3%(69/109)、36.7%(40/109),两组间分布差异也不显著(P=0.511,P>0.05)。结论MGMT84C>T和MG-MT135G>TSNPs与哈萨克族食管癌易感性可能无关联。

Objective To explore the association between polymorphisms of O^6 -methylguanine- DNA methyltransferase （ MGMT） at MGMT 84C 〉 T, MGMT 135G 〉 T sites and susceptibility of esophageal cancer in Kazak population. Methods Fifty - one patients with esophageal cancer and 109 controls of Kazak population were detected for SNPs genotype by PCR - RFLP based on case - control study. Results The frequencies of genotypes CC,CT,TT of MGMT 84C 〉 T site were 76.5% （39/51）, 17.6% （9/51）, 5.9% （3/51） respectively in cases and 77.0% （84/109）, 18.3% （20/109）, 4.6% （5/109） in controls, respectively. The proportion of genotypes for MGMT 135G 〉 T of cases was similar to that of controls （ P = 0. 938, P 〉 0.05 ）. The frequencies of genotypes CC, CT, Tr of MGMT 135G 〉 T were 90.2% （46/51）, 7.8% （4/51）, 2.0% （ 1/51 ）in cases and 82.6% （90/109）, 16.5% （ 18/109）, 0.9% （ 1/109 ） in controls, respectively. The proportion of genotypes of MGMT 135G 〉 T of cases was similar to that of the controls （P = 0. 295, P 〉 0.05 ）. Combined with the two SNPs, the frequencies of 0 allele variant and 1 -4 allele variants were 68.6% （35/51）, 31.4% （ 16/51） in cases and 63.3% （69/109）, 36.7% （40/109） in controls, respectively. The proportion of cases was similar to that of the controls（P =0. 511 ,P 〉0.05 ）. Conclusions The results suggest that there he no relationship between the two SNPs and the susceptibility of esophageal cancer in Kazak population.