摘要
为制备兔抗小麦钙调素抗血清并对其进行纯化,首先从小麦叶片中提取钙调素蛋白,对其初步纯化后免疫家兔,制备得到兔抗小麦钙调素抗血清,再以大肠杆菌中诱导表达的拟南芥钙调素蛋白作为抗原,利用PVDF膜结合法纯化得到了兔抗小麦钙调素抗血清,并通过Western Blotting对所纯化的多克隆抗体进行了鉴定。
To prepare and purify rabbit anti-wheat calmodulin antiserums, calmodulin protein was extracted from wheat leaves for immuned rabbits after preliminary purification. Then Arabidopsis thaliana calmodulin protein was used by E. coli induced expression as antigen to purify rabbit anti- wheat calmodulin antiserum by PVDF membrane binding. The purified polyclonal antibody was identified by the Western Blotting.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2008年第6期55-58,共4页
Journal of Hebei Agricultural University
基金
国家自然科学基金资助项目(No.30671244)
植物生理学与生物化学国家重点实验室开放课题资助项目(No.PPB04006)
河北省自然科学基金资助项目(No.303180No.C2005000220No.C2007000515)
