原位萃取发酵耦合工艺高产丁醇的初步研究

Preliminary study on coupling between extraction and acetone-butanol fermentation to increase butanol production

以粮食为原料通过发酵法生产丁醇是一种经典的方法。然而,发酵工艺中存在着缺陷:低浓度的丁醇会对生物系统产生强毒性,由此限制了丁醇的高产。而原位萃取发酵耦合(在发酵过程中采用了有机溶剂连续移走发酵产物以消除产物抑制作用)工艺,是解决上述缺陷的有效途径。本试验开展了原位萃取有机溶剂的筛选、配比的研究。结果表明:在所选的油-20%癸醇、油-40%癸醇、正辛醇、正庚醇、乙酸乙酯5种有机萃取剂中,其中油-20%癸醇,1∶5的配方对生产菌种毒性最低,丁醇总产量达19.21 g/L,而丁醇总生产强度也比传统发酵的对照组提高了62.8%,总溶剂的生产强度也相应提高了42.3%。

By knowing the biocompatibility of the five kinds of extractant system, such as oleyl- 20% decanol alcohol, oleyl - 40% decanol alcohol, 1 - octanol, 1 - heptanol and ethyl acetate, a mixed extractant that contains decanol in oleyl alcohol is chosen for its low toxicity to the strains. After measuring the extraction rate of oleyl - decanol alcohol, phase rate of 1 ： 5 and a mixed extract- ant that contained 20 % decanol in oleyl alcohol are combined to carry out in - situ extractive acetone - butanol fermentation, resulting 19.21 g/L of butanol concentration. Butanol productivity could be 62.8 % higher than that of control, meanwhile, comparing with the control, total organic solvent productivity increases by 42.3 %.