摘要
家蚕茧丝经高浓度氯化钙或碱溶液处理后,可制成不同形状的丝素.将这些丝素分别与果胶酶通过吸附以及与戊二醛交联结合,制备了不同形状固定化酶.对固定化酶性质的研究表明:丝素能有效地固定果胶酶;其最适pH值为4.0,比游离酶提高了0.5个单位;最适温度为60℃,比游离酶提高了10℃,改善了果胶酶的适应范围.同时还发现:在制备固定化酶时,戊二醛的浓度以0.25%为好;
After the silkworm cocoon was treated by high concentration solution of CaCl2 or alkali, it was utilized to produce different kinds of fibroins (fibroin fiber, fibroin powder and fibroin membrane) which could be as a support material for immobilization of pectate lyase by using glutaraldehyde and the sorption The experiment certified that the concentration of the glutaraldehyde in the immobilization should be controlled at 025%, and the content of the pactate lyase should be regulated at 3 mg/L And it also proved that the optimal pH of the pactate lyase immobilized is 40 and the free enzyme is 35,and the optimal temperature of the pactate lyase immobilized is 60 ℃ and the free enzyme is 50 ℃ Therefore, the fibroin was very useful as immobilized enzyme support material
基金
浙江省自然科学基金
关键词
蚕丝
固定化酶
果胶酶
家蚕
silk fibroin
immobilized enzyme
pectatelyase